	   SnapGene      #cccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcgccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaattctccccagctctttctgcgctgtccataatgaactccatcgatggatttcaggagtattggagtatgtggattcaaaacagtggctaattgagtcaggattacttaatattcgactcctaccctcagaatgttgttctccaacttcaagaaactgttacccatgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgacgcagtctatcggaggactggcgcgccgagtgaggggttgtgggctcttttattgagctcggggagcagaagcgcgcgaacagaagcgagaagcgaactgattggttagttcaaataaggcacagggtcatttcaggtccttggggcaccctggaaacatctgatggttctctagaaactgctgagggcgggaccgcatctggggaccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattctgctgtctctctgttcctaaccttgatctgaacttctctattctcagttatgtattttccatgccttgcaaaatggcgttacttaagctagcTTGCCAAACCTACAGGTGGGGTCTTTCATGGATCCAAGCTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTCGCGAATTCGCGGAGACTCTAGAGGGTATATAATGGAAGCTCGATTTCCAGCTTGGCATTCCGGTACTGTTGGTAAACACCAAGCTTGCCACCATGGAAGATGCCAAAAACATTAAGAAGGGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGTAATaattctagagtcggggcggccggccgcttcgagcagacatgaTaagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttGCGGGGAGGCGGCCCAAAGGGAGATCCGACTCGTCTGAGGGCGAAGGCGAAGACGCGGAAGAGGCCGCAGAGCCGGCAGCAGGCCGCGGGAAGGAAGGTCCGCTGGATTGAGGGCCGAAGGGACGTAGCAGAAGGACGTCCCGCGCAGAATCCAGGTGGCAACACAGGCGAGCAGCCATGGAAAGGACGTCAGCTTCCCCGACAACACCACGGAATTGTCAGTGCCCAACAGCCGAGCCCCTGTCCAGCAGCGGGCAAGGCAGGCGGCGATGAGTTCCGCCGTGGCAATAGGGAGGGGGAAAGCGAAAGTCCCGGAAAGGAGCTGACAGGTGGTGGCAATGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACAGTGCACACCACGCCACGTTGCCTGACAACGGGCCACAACTCCTCATAAAGAGACAGCAACCAGGATTTATACAAGGAGGAGAAAATGAAAGCCATACGGGAAGCAATAGCATGATACAAAGGCATTAAAGCAGCGTATCCACATAGCGTAAAAGGAGCAACATAGTTAAGAATACCAGTCAATCTTTCACAAATTTTGTAATCCAGAGGTTGATTttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatcggatcccgggcccgcggttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgtcgacgcggccgcgaattcaagcttctcgagggctagaggatggtccacccccggggtcggcagccttcacgtgggcggcgtgtatccaagctgcgatgccgtctactttgagggcggtgggggtggtcagcaggactgtgtaaggtcctttccagcgaggttctaggttcttagtctggtgtcggcggacccacactgtgtcgccgactcggtaagggtgaggtaccaccggtcggtccagttgttcttggtaggctgccgccagaggtctccagacttcgtgctggactaagtagagagcctgtaagtgagcttggagagaggggctgttagtaactcttgtcatgtcagggtcagggaagtttacaaggggcgggggtgccccatataagatctcatatggccatatgggggcgcctagagaaggagtgagggctggataaagggaggatcgaggcggggtcgaacgaggaggttcaagggggagagacggggcggatggaggaagaggaggcggaggcttagggtgtacaaagggcttgacccagggaggggggtcaaaagccaaggcttcccaggtcacgatgtaggggacctggtctgggtgtccatgcgggccaggtgaaaagaccttgatcttaacctgggtgatgaggtctcggttaaaggtgccgtctcgcggccatccgacgttaaaggttggccattctgcagagcagaaggtaacccaacgtctcttcttgacatctaccgactggttgtgagcgatccgctcgacatctttccagtgacctaaggtcaaacttaagggagtggtaacaggctagcccgggcccatattttcagacaaatacagaaacacagtcagacagagacaacacagaacgatgctgcagcagacaagacgcgcggcgcggcttcggtcccaaaccgaaagcaaaaattcagacggaggcgggaactgttttaggttctcgtctcctaccagaaccacatatccctcctctaaggggggtgcaccaaagagtcctaaacgatcgggattttaggactcaggtcgggccacaaaaacggcccccgaagtccctgggacgtctcccagggttgcggccgggtgttccgaactcgtcagttccaccacgggtccgccagatacagagctagttagctaactagtacagacgcaggcgcaaacattagatgccggcacaaacacgcacaaagacagagacagacacgaaacgaccgctggccagcttacctcccggcgggggggtcggtggtccctgggcaggggtctccaaatctcggtggaacctccaaatgaaagacccccgaggtgggcagtcaatcaatctgaggagaccctcccaaggatcagcgagtccacgattcggatgcaaacagcaagaggctttattgggaatacgggtacccgggcgacgcagtctatcggaggactggcgcgccgagtgaggggttgtgggctcttttattgagctcggggagcagaagcgcgcgaacagaagcgagaagcgaactgattggttagttcaaataaggcacagggtcatttcaggtccttggggcaccctggaaacatctgatggttctctagaaactgctgagggcgggaccgcatctggggaccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattctgctgtctctctgttcctaaccttgatctgaacttctctattctcagttatgtattttccatgccttgcaaaatggcgttacttaagctagcttgccaaacctacaggtggggtctttcatt  L	   D   &   2   ;   4   (   "   +   ;      "   *   B   0   ,   0   <   &      9   9   *      &   &         +   $   %      "   @   $                     #             #   "         =   %      1   5   *      %   >      1       8   0   "   2   5   2   -   )   '   .   !   &               2   *   7       :   3      (   )   4      /                  (   /      #                                           $         "      !   2   +         *      #      +   3         (   1   (   5   /   !   '      !      '         $   (   .                                 $         %      !            (                      6   *      /   0   !   (         "   *      )      '      '   $         !      6          5   ,   "      #   %            #           >   6   3      >   &   1      (   '         &             /   5   ,   '   -               2      &   6   "            .   )      -   5   $   '   %   &      '      (   #   $   (        D Þ )*0noˆµìíî’	“	ã	ä	å	¶Ñ#^zíbµ¾X|}¶·´P‰˜™èJKEJKmnž´µ¿ûüý!ÅÆÇÉ Ê Ë Ì á ˜!³!"@"\"ö"& ß +n«1p‹¶M	‡	”	ÒR_unc8Yh­Íx~-8�¶÷"ü�ã a!´!4"A"2 !n·ïo	³	0v‡·öRIµ¶¸µQgšf„éG	L«2LŸàþ.YÈ! , I Í â i!™!ó!÷"; è ö 4yã ‰ãæ	�¾$N{V�Å¥2î1r›¿|ÅRéò^}Š¾!'¢ñCFo„Àë:÷Ü !q! !"0"]"4  ¬HS’_·	N	z	ˆ	ê	¯·C Ó+Ig…£Áêbeþ�Ziy”eÎ}‘ª·ÈÑ×øýŠ$!‚!µ!( à o2>œŒt	•	ó	¢
¨
û
]Q-€.Ljˆ¦Ä>ðŽ®²	)�.9Ë‘ô b!"b"" 0óqÇA£ùÑQ
i§ÀÜf !š¾
ëÓ0žù‰”Ää2!G!+ d ‚ – ,K)¼X	£S`vÎod�£9?TP`qðÎÛ7b±ÇoXŽñ#ì …!5"B"; F · %#÷eæHê¤Ë(Sb˜‚—š¶?BVðž¡±h›g¦36W®ÌGM…¬Má/@ZoÉ- Ò ñ !ó" ø=Šw’ü]	d	Ÿ	1ˆ÷&ª{÷p¦ê¦±Í¥j!" ¼ ¾þ"ÏKí¸ð´	
ï
w¸ü:&¹›úv]ry­h�È�" š!Þ!"* ¯oßp	J·¶¶&R¬…äHý
Pcº¾Þ3 ÿ)bðmóJ Î Õ ã ô!þ!ø"B ® Ã 5yªåì…ç¢×U	ç	�¨OñDb€ž¼Ú~¦êïqÅ(ÓœÀ]}ƒü\_ #_°ÐÄÏ D‹Gp›ÁÔb�v Æ r!Š!1"Ó"0 ¥ ‰&Ð¡÷Ë ¾k	æ
/%|«øž‘å!&ziŸ óSXq¤D~¿"(Z£‰ Ý !í!"^", - < é ÷ zCG•ä8MÎ¿ôgÆF3B2‘éýYsðòÑ…Úì	gX!¡!0 ¡ %ºä/:ŠT

#
Ñ
oœWm
8×¾’ÃÇ`”§Æêó‹û€Úe;|‘ø ( ¶ Q"~"< ¸¼I…	 
At¡´L,Jh†¤ÂôÉ`cÿ�û=T[z’Ãz™ï÷Ap¯Ü‚’™¨«ÉÏÒØù`‹!"!V!ƒ!–!."&  ÚsIà­	6	O	{	DYìëç2aÞŽ„jýÏûx¸º;Ÿ %!Î! R  ¦KÓ ¸vg
”
°¸Ö°ÞO¹êe~þÔ:!’!9 ^ þ ­l¦T“®`h† (	/	}	‰	È	ë	k
‹
œ
¬
	eËÔu�ì†Œ»cf¶ÈåC`�ÖÐ›ÿ@•f³ý	�ÎY ­!¶!W"9 á µ�
XPœKJ	£
©
ÙçîA_}›¹×?So•üâ_]5˜€†�¯³ÊØ/ÚC� ÍÐô:zÌ¢ »!É!Ð!* pÑß3–ïúåÏ—8	Q	–	ô	ü
.C/Mk‰§ÅÁA	
€†�"C+xª !"%" áèÆ˜^úâ4 é*uàc!& ?+[�êËbè		u	ƒ
šRâó�Ä”‡»ˆñ,s?x±¤F’¯5 õ Ä!Õ!c"¦"& Û ó?iô¼�ÈØ	¤g7BéÍç&b•Èæ1IX£<Ÿú2Å]Jƒ H!" I l ì
�$VâX/¥ÇÒ!	R
p
j€�Úß<ŠS r" ñ!%Ï	I
Õ
¨ÁÝø	›È††¿ŸåB!Ú!+ ä Ä1BDr„ÙBNœ¤¯ú¿!"Ðë÷�í;àUßf}¤lì@Ô[•NÒß3!¡"$ — lvÜš«²Ä-“r½e:@¯ÇÊQr‰ÏÜ²Õ¼„[‡ p u!% Ò X—uü>´ÕY	ë
Ïzp÷Ã=h¤¯+ÕñšãÿpºÛ!7=V› ¡ ¥   ƒ -jÊ½Ã	÷
ì¤í3U~žaqÑcÈY¡æòÄí†!" e ¡L*cŽ	‡
#F[TawjÙÌUe8™�ñ$Hé¯ !'!6"C"@ Ÿ #$ƒlë“ O
[¾Ì¼Geƒ¡¿Ì&ek�	€˜›ÕR@NñoŸ¢´²tªÎâ4X~ÍHN Ó ò !ž!ô"$ G hŸêø"²fIt-	Ö	i
š
c²¹1¹$>¯­€A[Ìç”! ¶·E­Y)McÂ·CH«œh#]†™â0.  ¸ &8çõ¥+TÚ[N™ƒÍëW3Ìi(§Ö7ìNpÊ Ã¤”GMi2Œ©w|Òq§‹µëTÎø ­þ> x“ýÁe	'«ø­8_‘_§ ¦ ùg¢¨‹•xŸ^	 	Y
R²ºÚø³R%¼" 2 1Õ‰à'Q•VÈ²äk k!# O ½ 1ÕÐˆÔ#¹Î
Vþ¹#OEnSºÜœMwË
4ŽÄŽÉ# ›! |Jÿ#§PŒË?
ýÕôï'pûi‡_ ß!·"Ö" [¿KàM_Lxîòµ	ð
x˜7Us‘¯Íì„=—z"  y~<ñ
3
;i³ð@[&JgžÝì^szÇ®ž0 ? ã!"K"•"# Xe¹°ªàq	°	Ì	cÝ°K¸åè»z�­øFj”ÓåŠÒˆ›»¡ôÏ " T ¸ÈßºŠß	–
Ø5Î/¿}'IQ).4 ñ3; !õ! t ¥p¯™«v·S†8�ß*cu°¤ ä {!�"ù" «	‘ÄÑ·ßHŠÂ:—�þd¿2nÖ K Ö <!ÿ!= º ö<Î;ˆ-uèÝV	i	è	
¦
 gPè@èëÃç&=�ÁV^Œ«P]ø!$`Ñ[‚ÅîØEŒHœÂÕ8z`eŽ& Ç 2"% ³æØè£
	’
ž
W‘àêñà0Š×Æ)Ô‚~„ý`„±Þ0nÐ8!s!Â!Ì!Ó! z«°jˆ¿+	Ê	=
Ì
òEc�Ÿ½Ûî§Ï½P]Ð!Ô"1 ¯ Ä  6SØíoÖ:†×4½*Ø<		�
®
Ê
Œ©«ÜÞ]˜ˆqðKrÁ;äD€©q^cw ‹!�!¾!À!5 ‘Ú&€%,Úl	}
�
à
–Ö}ƒù-=’stâÒ˜¡¤ÕæMYM4‚)9UšŸPSs Þ !x!¸!_"* ¢¨ÆÌÙ¿Ïÿ	¹
jpê½ÊÜæzÓú{œjy HôTr¥Ã2h|y K!R!Ì" š ¦ CEŠ²Ñ¶êø÷X™Ÿ#"'’8EÀ[:"% G”¿('neƒl÷�	ç
0&‰¬À´¹¸Ú!EÎ…¸÷ü#¤ÌŠ ” î!"> C À §iœ¯ô•DHSÝÈ–™å 
m
ã
Ž*I¨(†õhŽ‘Ç/•ÊZ™Øí8E»—£µ¼Øñu-Òî†M³ +"Š" ~Váƒ1	Ïýšÿ	tåý4h±3’êþt)ó
[ c ß"1 = {�ˆ]ùË	C	‹	9G†29Ge¾C[v©Ë/Fô$Û,MèÛíh» Y!h"ã"  . ê ø :ƒmæ6~Û	+
v
#NƒÀÍPõYkT«•¶Ã×¢!¯!8 “ ¢ Ï &UB�³böJz
€
Ò
­w{Ûò…²»Ðß¨ÄÈ•�¨Ç=lŒ’¿é7‘ 0Ql��Û5žÕr) † �!Y"0 ± î 8£O_0Ÿ‹Uœ>	º	û	6E_�¥?ä-M"0“×ë&¥üØf<}’ùO · ç"" ` ‹ Æ ŒÝïåñØ¿éq›	í	

$
a
À
å´9m1b¿Yß¸ÿsÇ"2 »ÈSq¼S;—þ°
p�XŸ§IVn©]|Ÿ¬Ó�›…ØÃÛua5Bªô*Lm`f½P R""5 âL	n	†	‘	â	uµMõUÄ¤0aqšg{ÄÌw{]e
 FðBª17Dƒ�³ßêöú ûÄ  à —!ò!/"2 ›JR(@¢y	¡
®ÛBP¢-Ki‡¥Ãadý>
_±Ú“	d6/�øW|“©ÊÐë#!1!„!a"- ¶ ½ý"åG\	î
Ê ~–z%Uµï�š°oVbqx°Ý¬?anŒ¤ïœò!ý!ò") ; ¹½SÖT	.7
Ca�»Ù	%y�ü‘\‚“¦ûW[CÐšÓÙŒu W!ì!' ] ·»ŸJ¥ÿ'|	«
@sóbµ_�¸‘šü˜öÛúüw¹Îœ:ÓX ž U!. îZÅä–a7	P	ùØæíB@^|š¸ÖRû3Àá@4—ÿ×ètŸÏy¡*E®º!È!Ï!$"! ã AŽØá M›®. 	�AèÏöì…ß|…£Þk¢;ZR ‚ q"&  –ÛtÔ�Ã"EZìq}®ÔˆþÐå»G 6<UZ†o š   ¤ !&! !D’Ÿh
™
Ë±Ž2³Ës©æÿ“! 0¡§ýÔÿ±¹ßP‹”Çv÷j  ÔILx"¦wOÊ Ô?mëRfoÜ�Ã<> ^ â!¶" S Þ¹©ª	•
×4b˜Íäº‰|EÒ€2Õ !;!z!2 ÿ ²:¯i‡ç)Ü		*	;	h	~	É	Œ
‘
�
¥
­
ÿ

fVÛéð\—í/‡çJÀæ%OƒO\ímÏ7!½!Ë!Ò!* “m§éÅ‚ö$kþ	Õ‚˜¼"·ÒùD‘Ñ£Ô LÂ17Lg3�„öTžR{Q!·!7 B ¦›®U”ø	0	Š	l
â
"‚Ìü '1…þ8s�ädgu¨É×7D–´#S+L”µç,
Z b º ®!Þ"â"  _ ÜNRža–Iýì	
v�¤¨«la~„!œ<A%·þX": ášQ?¡ÕK	S	O	B`~œºØTÃ£ü`}•p	^�™f�°´ËÙV’Bca©C{Ž²é
m‹£îúÃ0!ë!3 â ¶ž@Y¤Lšþ`Âª
!r@Qp¿Îàõ
|„´­^3{–¢Ý‡�ûsž¡ÎYÍFYÒ™ !T! üw!¨aÆlQˆÛDÑö; ( Mšè�•õ:	g	¤
þ
Úèïû&0Ž–ýã!Ñk6C‚$0Kõæ+z¼!Ê!Ñ!Ý") à™ûvç >™ 9	R	ý
 �ÂÍßô ]e�‡‘ú#AJb�èElŠyÑ˜ !4  W q—˜ æ*=g˜ÕÖ×Øõ	ö	Æì01NOlmŠ‹¨©ÆÇHIÌÞósú
aÙD~þÿÎ"   } 4Wa„YÐh5íjŠÕÚ$€ê ý ç!Ï"/ X rÒð§Û+ÑÙ—	÷	»
Ç/D2PnŒªÈ)JtBûôMÅ¨ô,sÛ  { Ž « M!"&"ê" âé5…fÇ™ÈÝ
—ûîþ§þ Ð"  ¯Xbx��
Ñ6´
å5k¯Jt4vÖ%d!è! ~ 8_pxÒã²+‹á�  ‡ ˆ5Z[iÃ†8‹ê€“Ûë !! bÓñ\A¨ÑÌqé	˜	ø	
„
wƒ×Læ¥­GÜ ¬ ( z Ñb®7'iÎÒv	mÈSã0*K•ˆCòž¦òNö!9Æj²-@| � N!Å!"/ Y ƒ,ª3zÜãëÁÿ,c	›lôE‚Å3Qo�«ÉÕ‰ûð-t@y"õ“6 f Ö!'"d"§" s@{YŽÚU
¼
¹	uèF¼õü,õ:¯©T¨t°ö ›"ë"# + Ü ãê#õlÉ�Åñ	¥8ïÿ<›–¤Â ÊûckÆ"Ñ" ü q¤gjÈúšÉa4	˜“ê…Ýã Øèc :ÿ=^ÿ  ´6e�v1
T¼üØ%$T<É2à,º" Eô@†½Ù	Þ
EhãCGÑÎ'¨çJY36K„ I!Å" 9 °GûÒãÈ¨	6u5Zm  y‚Ž%WY¦ÓS
k•ü‚°ÛAKø×W‹é! J m Ê !c‘æ‘¡úÄUai¶s"ƒ" íY¸¢07w"	q
µ
�<Zx–´Òïlà)=w&T e! L  “—P}ù"`´Ó|Èùœä,À,^!ª!Û! �àò[
Ú
+ÞNÒ�>G  " ûõGO±Ó&29Ð	q©Ü
;¤ 4Œ ãæø!¾" L]ªÂ¿J
Ö
yÂè£ÎäbµÉk‡P‡³¾â•‚C!…"°"  ˆ œ Ì å ‰Å(E…¡Ú¡�±Æ#_yÀá.O¥èA]–à! ¨ Õ Ësz�û9¡ìøûþ‚îVàg!4!$  ' ;
62ì[ªC\¥Ä¢	3ZeÀ}ß<Œ©�ÜÕ\O½Þì !"<"G"¢" o ˜ŒiC�Oj°‡`	B
“"Ññ )”~mëT”í'€Ó¸u" wñ³ê
.…s6õ'ç+‡%½ˆ G g!" ÝB©¬º°ÍÅ	ƒ	…
é
x„MSÊÜf;AÈËR*¦HÝ¨ ­ @! c]ÒrØíÐÝÚ³ˆ…® ! ‘ ˜ mÔïò›A	™	ù	
·
”�¾Ù-°sŠÕºþsÖ\q ’ v!2 ´ Ò”8âýÖû(	Z	w	ì
ÉTä�>\z˜¶Ô–‰>#ù:›É ®³(08CV m œ ¢ O!Æ!ð!o"ð"+ { `3˜v?gÏÓnv_‹�ñqÄDión*òùOäî?ˆAæ">WüŒ –  Ó –[¯&ju/
1LÂÐ{,§ÖâXk»Ü´} " Ycº(µ®ù	>ƒ+)øŸ¥°Ÿó÷"ÇÐq”.A� ¦ * i .k-*ä…î ƒ9	ø
m¥õFƒ4RpŽ¬Ê ²5ŠbdùZ–Å”‡!×!("e"  Z „ ¼ÝìÂ¡üÒóg # ñ „)pË48ï.-[Ä	@á	Æ÷â4V7ÖŸGþuÉAz<çö¨"Ù" 4 *«×{ó	d¾'œí<îkºrñ.¢#î7 + « Ø Á¢ÅÎMZ�Þn´Î	V
^
s
ˆ
½
Uxº­èk—±¶»úX§V-f·Áu° 7"3 f IÛ¤·+dÛä2X�\bŠ-9®vé*"G½wýö;$°Òy9bš¦Ç´«±ÎD D"l" y	$	½	º
Õ+Íªáz%IUê¿ ÷ œ"ì" t[eA|ê�2ó$G‹(gBÚYö¡©�ò©!(!m!
"( Ý 3m mŠm‡½"]Qœ1´7µ³Of—¡Il-X Û !`!Ÿ!"?"õ"1  c � /^Æ»	ò	P
ú
\¦¶¿~,*Hf„¢À =Íœ¢SŒïOpï½Í°ðˆÇó "( E $öÎJ‘ûìž	û9%£'
—�™A u¥«¥ã5ËüO„Ì(ÇÔ Ý!"5 ,   Â $x©äë%„öÊæF”¡
"
Ð
å
Í§n›ð÷fl�pÖòp£ÏÃYÎd�µ ‰!P"}"Ò"/ Q ý r¥HkÒgßu…¬	.	5	Ç	f
j
›
dÕ³ÊKtëºäéyÂd@®�_Í!!•!¬!-"V"! à•ù	—›ÊJç™Ã”“†º\!ÙBœÌó>w°B !¥"' H k ò>ë	€h#ƒUð$W×	H
o
÷6¾€:TÞeÙæ%;ž?å M\Ù!" " iku ™Éû=³bÂ	,2<d™âð¹è! žµ7‚Õ	N
XZb½»*djÁ¸ÌG#=Í'á}\˜Ë�! fwÂFhÙ&U¬7�Š$^ã»"' N 0Jßñ;‹2
Uý h—²6Tr�®Ì"NZ%DI�Ê3Æ–/ J"y"”" s ~·¸®¯	�ªÃ.Gy÷i‡š(-1Œ" ¹ R×õ9Í,Ö3¼<
‹?pÎ:U¼Z¨/7% $ BF�'±d%Îöß
ioéˆ,<ÉÛyÙ ›GÍ·û8™OËJ!Ë"( ¿ 9‚‡å}RÇÊ‚˜Ú	
*
FH…§�O”˜ì(.Xj¢ó×ÚÂíL*"g"‰". A×¾èõ›ä5D¦HU>\žÒ�š„ÂÏÚX4©Þ¤¾è)6K�/P4ÔOq… Æ"æ" : üäFR-xvUwÏÞ›ê \ Ó•Aò·þõùv� #" H¥CNÉ©	3ÿ�nr¨Œ] i á! ±Ä÷Hj!ÿ7ª§}Ó6[¶ìa á" L?”ô§Ôf	úPƒ³Ð”¬�ÜBr`¨õXíùÂê!Ü"   VƒÿuX)<(ù\LùI`ÔØŒk‰Ú † ‡®�	7ZÂ…Ü
–Ñäý®±’ y z&XyâþpT
l¸vñ9!S§e š" * "d£€
Ä3	ð	DS»×bj¹" 8 É ú§	ùû�@V$ ! K úôFN’©ÁŒ–¾ßOZ
³ç{Ç¢ÍÛ´j£+3â]!©!„"¯"½" & n Ë h�y ©_	¡	A
œ’ÅÿSç&·~!t" � îð
¶
œØRÉ5ì*‡f!% _2“Z%¹­t	'	=‚=[y—µÓ€ð( m)á>­@å'U l ï" 3 Ö7îò8àöá64ý;Ø"! ~£ã1xŽ³Í#	]
r
òŠ‰f¹Ôç–°W!vx³Àª¾ l!k" € 2Í‰úº	Ï
µ}ú!mš$P¡oîNË�_!Ü!"O"|" P ”G˜Ñ‚#V«Æ	e
ÔÉ½9åÁ�ØäA›Ë?ïL¸«!Ÿ" M }IðÔ	WaÿºÀF"6-� xÌö&‰|Å'Êœ!( ¾ AQ�ÖÌÖäcÕ$Q~Íô
)
è4;=�oxØ-T»Ý£W5�.OÁ$ Ê"å" Q ,þ¦~Ýšé` € à! x †K táó(6;oÛ
ãùOðŽHqRjˆøÛ"   % ùMÀŽ	N¨¦	@
CzÆÖ2?áæ\!¨!¸" ^}’$ï6ñ�²Ì
\
<ßˆõ¸Ó(ü†?½ j"×" HËÕãbUïóÓ	
(
d
™<í!5Ž¢V>{"   $ w ]øLsM'5:¥	
;ByÅ·¥1àå…>™è[!§!6    # \üÀö÷+PQÈÉÊÔáâN`r_`a34STy´µ¶·Ñ	Ò	



'
ÄÝ�¡ÇÈßä˜ç¦!¿"*  ŸýÁ,R²Oaz¸¶	7
ñ
r‡ªÞ8Vt’°ÎE„ºÉháù!À"ü" ó  -P{ò·	éåäñ¶.XgâF]®Œ¬KÍ»éjD!/ ? Â^Š«/!]×
s«Ã9Wu“±ÏD\IÊ'ˆL…»ÞP_wÝCã¯ƒä!ú!ý"0 	 þSzÀ:0¨¹=@4
K
zˆ<j¤ÈÏcAl«Q‰Iˆ´ÊHŸíðêt{È–µŸ"L"†"ª"±"! MÌ³ÃÛbû?E	�	
8
ò
I´ß6h¿iâ1 @ –"Á"( æ ô 	.­F8ár	±	džÏò`ãG¼é{®ù}¼æ‰¤¼fkáõÐ ï E!€!±! )Í	¢êmÇåh^Á�­ÎÆGVq—Óí—¢Ù 0 � Yfè†¢|«óÝ	P$±·/YLPæéLt	K•Ôê¢9 " ‰ Í ì Š¡ÆºQ±}sÛ|¸	x
Þæ²z¯â/¹¦B�k^dj‹»œx* @ .¹ Ã»‹"à	—
¬Ù÷>Ò¢Ë÷0À[mQDJR/54„ð< 5!û!´" U _‹¬5žü^ÀÄ6ÕJ‰h~ä°ò!!å!) ú Éot{0_Ø
):Xv”²ÐE]íùüÿƒÏïR¾áM‹ö¼Þ*_ö!þ" © Ö ÃÌèÙàì-
%t:(W†(ß`xÅ' 
 7<€{;1\ºÅf€À	L
‰4[f¥°Á=�RwË ñ‚+—!"="H"‡"’"£" ( 6 Ž Tõ«DAš?Ëðd~µ9u¾À|!!"­"'  u í¦©>°£	5
…ÂkBàm¬ŠJª‚¸ÝTÖî‡|É%P³¶ß¤!M"ú"$ <ÿ,¦3Áq]Ã
{=¬®ÉÐ¼	‰Iëà]dv± ¥ å í "Ž"�"«"²" �¨j´@a	JÅ*Òn¸i�Ô2 L  c€Ž	’žà#ìIU•0 ‘ÿ(jÀâ3ãA ç ú =!Â"! p >NÜDP±ˆ¬	
9
ï”µÒ•óÃ;>˜­¸e§½× "v"—" ™Í…Ä‘ü›kF	C
Å
ó
}K7òà‹Àîo¹  Â × ,!6 ç õ (xª/Ÿ´¶âë²	/†ŽÐQytmHì´½W¬Qè,ñ|ˆƒ½ç&I�¾9�âö+ H È h!p!²!3"["  • =òG‘øÐW	s	é	§
heŸé ™�(ŽêaÒÆn�ÃÖ‰F!�! » ÷<‰®dvéÞc	
a©ö>ù%\2­F¥¹½glÑ ð 5 ¤ ­ 
ˆÏ.ÿ9Bj	
L}ªóEä Aé7Äè'X½ÒhžÂÆ_Ÿ^"¯ú~ïÙŠ{af ' ˆ Å , Ù­„	Š
“
Ÿ
X¯ë€ÈÝæ1…‹N`_ÝŽÇú<BÕSƒÏþ?²îo˜§!9!‘!Í!" ´çÐÞ*WOéÎ		I	‚
áòán^‡…É…+rß£w
 4 © Ã!Ô! Ú ÃC»¤ÆÎ	Ô
£êÇÌa”ÇHW1ÑIÞA!# Ñ Wª±	†
ê
ö
ë’¼yTiØöÂËg*®ÆÉp˜Ô ƒÃÚ® t!% ž "gé¶±ks¬ô,	Fd‚ ¾%L¸Ù0ZMÔQêMªnÕ"ë 1 ¬”ž£“ÀLX
Q²¨ÑQ^´S Z{^‡Ó}>
Í
óïîÛLë	ƒ†Õ" Wd¤Ç‰Ë	Þ	Ü¯ÐçuÞ¾
ŽÁ9“–‰�Ñ7t¯£ : # nÕ×;‡t¾
É
Ë
ªÝßßç‰ÖÂ<�‹ªã÷C�×Ýy]_dŒ!¿!Á!  ™ ¾ÙDµØ+Ù€	|
Ž
¸
•W¿³¸rsáx—år x “ w!9"  h�ló\5Üà}B	u
�)™½ü.Z°ôÊºE»ªtÖ² > Š ’ ° Å Î í 7T‹¢Çî^pð»Rœ²~p=	š	¹	ú	`
y
¯
¿
¬^zž³Ú{ãñ,L0±ºÞ/t§kÖ‘lÿ_ekŒ¼�×rN Ž!6 µ !'¼[	m	x	�	á	í
6­Ú~$/y$¥îúZœ¯5\n.d	EœV06õ>ñ t ß ñ!ü!`"ñ"3 •ºíÄã�×Œ&-ø?×åŽ?]{™·Õúë?Ó™Öç—:Úä› )59DT…­Q W n � £ ¹!Ç!p" Ó/Ýþ‘ž˜
Ê—Ó>Š“Ìuã1²Ê{Âå1= !y!µ"> A ’¥ÛQ9�æ#Ûü	)	ý	~
�
á
�U[„.r—£§tæøI¿$ƒ ¢;KNÁN2€ƒ"R*K“´SlQ¹ 6!P!& ™£
ý_ÁN`q%/¾	¢{Åûj|o2kz•¡PUå*B±	X/!S!1 V | `¦ÚfŸÐÔ4º
ÅëGŒËÝòriódÄ@‰§çó#r}Ðýz � — é ü L!æ!Í"é" 4wŒeÇ³
ow7ŠIs¦3!!( a‚Ða­6©2@hÍÐÀ 
k‚ÖKçÔEô�¥+úõ 8®åïi¬?' û D³pùku`0
â;’Fš $„Ð#SÜâÿ×9;þÁßÉ+5Ä"  F·¡v” ;Yw•³ÑÃî÷(YlT`hµ‚" K\°Ò|ø1Ù
*MaÑ:OŒã=F½”~ ÷!ÿ"& › § Ô :—	‹Ê'ë 2Yd°^| úýxÞð¿(-N“¨\¼Ý";"F" ¹¯@	‚	è
„,Ûô&ÔÙ)†¹ý$rF ‘ § ?!  ³ •áføú.
u^ŒŠÁž"øøÈÏíW‡“/B³û‹ • ï!n" h ð )(o»)-„í‚Z&?;j ¹±Fø•/ ª × HÀZdÄÍéÚ¶ÝWm	¼	1',8­¬*Aµºù)X¦ #Ñàay¥¨Æ¶ÍC 	"5  D b Á lw¨õ.åEI]†�	
!
ä
ù
¦+\)¢ö0–o³6‹î¼Ìe¤¯ƒï‡',WÆ ´ Ú ˆ!">", j ßñ=jt<–TÞï„æ	Á	G
n
+ö5JsÂ’;S[dcx˜áòv !Ø!","U"¤"' r /�ÞÁ:gŠ®	M
�g–©±5Sq�­Ë!YiËF[—•I"x"‹"“"- 8�°|+2»ÆÉ�—š;
ŠnG„¦‡�È>Ž“—šÁÙ9F¡¶½òÙú.Ó˜N)"f"ˆ" [ öEB@þñ¶ýôvu\ h ""à"  … UW„Ð·u²Ï“[ÓôìÁÙd ™" ) 7 È óE½ÞÃ2	ï	›Ì 
þi€¢*}!®" � í¬âs	&	ñe›×æ¯5 4ëÿ3u¬:¿©î"2 @PŽ|FÌ�ªÌ	Å	 l¼ç3:¿8nw× ÿ×ãõ%@ˆšÊ-N{ÀÊ&îK·"N"ž"É"ä" …‘¿î5ðn±â‡ø}ÜQ÷¼ i"Ú"&   v ž*Çq^R³¤		

&
6
c
:A†Ã¶D0ƒ¹ÆÞU=gàZ!¥!û"6 > ò Ë‰Ú9ú/>.§ ?\D	Œ	H
ÇãC-5HWfá\ª­ˆ‹HK«ÌGïOvºÜBéè´i©"" / ë ç¬|7r{Ü	w
OÎlsJÅUp|ci–ºì£ewØ¡8 î °! ù -nç4ÂØë^¿,
$(ö=ÑÔQ½ö
ŠõZlÄ^ï³" 5 � +;ô
e¿	Â
„Á>­¯Êï»$²¿£! "�"‘"¬" = „§šÄ
|�îJò/=¬·á¦¼ Á æ ù +!. ” £ ¬ '‡©ž�þAc÷µÏb	K�x|©Dl˜¨W³¼ÑgÅž�ð1m¬¸* ‡ Ä o!Z") Ð Ù ÂVÏVN	H	�
‰
Ó
õ
®»Üé_Æõ]ù†ÅoÈ>“Ÿ‚Âv	 3 
!�! !V£Æµ“�’KW
K®Ó©m�Àê8’R‚Ž6s- ½C]gŽk´[ßo_
t
{
¾
È
(Vy²·¼ÕûYó+Ýà.w€É–©¹âjöÜÖÕM ± 8"5 ¤PìÜŒå�� 	�	ü	5€Zq¦êå#.H¾x‚#íJ±z–1!-c’›kÁÙãô=ä¬ P ¸ $ �Ï`d1¥	e3²
M$.F¡¤1”c7­¦)«qÏè û .!è"' 9J “ŸcŸá:`‘¯9wÝNÛþ<':ýÈSg4~“E"�"Ã"% g ² ï ¸,�ÜVY?	»	]‹7i«@+�÷%ÓØìV§ ’µ²úB E >!m"& a q vÞðs~ÝÀ‰œ	­	
:
F
*r•¡X~’:nc–ÀEà® &V Ù "T"w" Ç òDr	%	î	ð¶Ö®Î2Z«të¨Ø˜"í"'  ?O��Ê¾ÆÙE€=Qm²

%
b
»æµ,‡Åâ?N™¹¿fJ"�"È" Œ æ™êz¾	Á
õIÓôÄ<o{bh¹»¾À ø *!(  ¼U\f†U’ýœ‘G	Ç
ô
'Œ¨JC^Ôô\øÄÜváŒïõ« Ã 	!n!# €Îëÿ±
4LžYhpª8 ÜœÚ�£b6¬,‘Róªp -!€"$  uœÉrC}½Å<˜lE
ôH)q W}­‘†DÄMnag¾º Ø )!"S"(     š›TBT�D
Æ
3~%&oÛóZ[Ù÷€¢C«ÁÂÃ+ªQ  !!  5Ž  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        >                                                                                                                                                                                                                                                                                                              -                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                         	         2                           6                                                                                 	         =                                        	                     
                      %                                                                                 
            &   .      	               	            E   9               	         	                           
                            
         	            
            #   -   =      7   1      +   0   .   '   ³   c      $         <      ‹                     >                                                                                                                                            -                                                                                                                                                                                                                                                                                                                            
               	   2         6                             	   =              	       
        %                             
    & .  	     	    E 9     	   	         
          
   	    
    # - =  7 1  + 0 . ' ³ c  $   <  ‹   Œ¿  ŽM  Žy  Žß  •7  œS  ¤½vÃ	¯­±“  !£Kb	

È
'(H]®VqrJÜYåZ[ž©J­à«Å{ µgtóô&s¾À Ã Ä Ø Ù è !	!)!>!�!8"S"T"í"²-[é*a¨Æ{’¯`B"‘~`"“$u![ƒ€ÆA!¨¸[×	Ý;í÷÷½
}_"t¦ ± Æ ¿Ýæí;¿û	m
°
0ƒÍ}0ˆÜhqrÁÆÏþ²yŒ˜ôPTÎî÷#0l¯!_""Ûl ²-[]él h²-[@™él ²-[é°¶F^ú*Üàa¨Æ¡
"Õ +s±v"C – ¨Š!a #�ßØa #:Ãú�ßÔßzª zÑëI+"õrÎT"I’YkÛ+"©
]¥w%âUb"ý!UD¿l¡"Réá 
K¤ó  Ø ¨‹­­b"ý!?Ü Ò8×Öpˆ±j!Ôß® Ã 5ìæ/ˆ%¤«S˜ï‚V£±Mpoj!"kt
óÕeå\Ñ  $.Ð
ÂÆúÙd{�' µ âŠà€[ò>  OB® Ã 5ìšÚï¯rÜÏp ÿ —ø÷Ú"/
§·g &lõËN¶CÌiÊ­!&õÌÊŒÎ^øïì	!n!°!VòÚ
ý
ß"&õ$V¥T[™ƒ3Ìò(!¢NÊ"d�µ  ëè ö ¾ËV@ò !­!¡‚	Ûç® Ã 5ìïpo†Cg^	R%€TÞek…²5d7 Ág&	¬˜Nî"® Ã ÿ 5ì;	­
\—ïJpZ® Ã 5ì>Œ_ˆæê	
/ˆ%R_ Vu¤«Sm˜ï‚×Vëò‰£Ž±Mp"om I Õ ô j!"4"A" ])‹Ì/áFªÅ	¿ÃCÞoÞbã DY%!!'u�	4MŒ)x: ¡.Óù³©%mïö,c{ªÈóôV    E Ø Ù 
!.!n!""Z" �Îa	—§·Æ¥|¢ « b{§V»ÐfÄ�0·† ©Ü‹!¾!A`c¨Ï"!ò·¼ÂÆÙ' ÿYp�/+7‡¶úÃ®CÞ†
iÂÆÙ¹Þ' ® ¤t¯£ Úº:ÈÞè ö &lõ¾Ë$V¥T[N™¶ƒC3@Ìòi(!¢NÊ !­!"&ãú o_ƒá/
M:¡È.!"²µ�©
]�Ì¢ À ê C£§»ODËû	 
v
ã
 ©Üý2{õ9Š¥ÓväÆ»¿ÐÔÛuÄ�¤ýPYô±¸ü,M8
s † ³ · ‹!¾!ß"ã"!:Ó©%möÈE ïC²/
#"0AAM£® Ï|këÓZ`0°x-\)øŒïn!©ös':U	ç	_ÐÄ‹ÁÔ*iL Ë­!l �r" ])[‹Ìò>  /OBá#¢K2€SQÆ—G¦�“—¡)"ˆ"âà `hïÒ0æ&õ'Ì'&›¹ïRNÌÝ^„¯Òþ8=âÊ<!
 ú fèo\_©ü^ÀF	ó
¬Ù?Þðå·/ƒáµ•Ë(Öëâ¶á!"n�¹q	cn¢ {»ÐÄ�† A ¥g9æÛá
(„æ$©¹Nö± ­Û=�!7|ì˜NA ¥á
„ÒFª¿Å	øì÷Ú"�å•L‡
é­¼kQ´f-X0Ôß&ãj 9ÍûÝ!?ðµ!"0qA£ù !ëÓ”2!BN¯!Ð}lìÒa v�	œ	
5*¡#.nx#í-c®›ô&=V  Ù "¥p«ßcu°¤ ä �" ÚsDYëýÏº;Ÿ %!‘Ú¯€È–™Ú}
�
à
ƒ†-ŽÇs™Ø8E¡MM‚µ¼Ø)-ÒMPd ºãK):‰S`oœVWvm×T`¶Åéòó7ŠýcŽ¾#:m÷ J Õ þ!5"B"Q"~"'†©žýµŒ�x¨Cl³Œïð«* Ã n!o!Z"F ÷eH®¬@Z© Ö ÃÌèÙ(Wß`xÅd K)S`vT¶7ýcŽ¾#mJ Õ þ!5"B"‘Ú€Ú}
�
à
ƒ-s¡MM‚)P¯È–™†ŽÇ™Ø8Eµ¼Ø-ÒM9Ÿc¯wÝ~E"Û ô�¤7•£ŸúÅ"6 UJ¨lŒ¿é7‘ Q��5r‘ÚB¯Î³-€È–™Úi	
z
}
�
à
ƒ†-ŽÇ@sèòÃç&™Øß8�ÁÈE^•¡¨M]M!‚µ¼Øî)ØÛ-ÒÕzMP`e& a 'v†©žýµ�	œ	
5Œ�*x¡¨Cl#.nx³#íŒïð-c®›«ô&=V  * Ã Ù n!o!"Z"š ¦ ŠêXŸ'’[:"ó?¼Ø	gBÍ&æIX2Jƒ H!k-ä…ø
¥²5ŠdÅ‡!Ü��±zÁäóÇf0žùÄG!—‹ð(“-Âö=ÑöZlï³"Þ„	Jsx !,"U"+R_unc8Í" 4"A"ßŠn Ö .Ò¢Åý 9
V
É4RpŽ¬Ê ­‰õÉZ–0”-ÂÞ„	ö=JsÑ²Ïö“xZl}ôÜìÁï !,"U"³"‘ž˜
Ê1²Êåh )„í‚±ø•— lš“½¯r‰Õ[p u!ˆÊ	ÛÏ½Ð¼\fÇ
'ÔÜváõ PëV«P‚Hœ8ŽÇ 2"R ¸½I
”
Õ
ÁÖìíÈ††¹qé¡Ôí:!B!Wdª±Ë	’Ü¼¯®ÆÉçŽ“‰ÑÔƒt!<¹Ã;F‹r	À	÷>dž4ÁÅRnwé�4„2 !E!€!"£"ÊÙ=,‡N¹PJ	A_}›¹×S_˜€¯³ÊØz¢h|Ÿ#ßx²ýŒËô1'8û' ¦çÖ"á µ�
XK©
?o]†�� ÍÌ¦>Bà¬ŠJªî³R ¸”
Ö¹Ô:!½ìíq¡íhŸß²1'çI
Õ
ÁÈ††éB!BÎ³-i	
z
@èòÃç&ß�ÁÈ^•¨]!îØÛÕz`e& h )„í‚‘ž˜
Ê±1²Êø•åšÉ˜“… =ÿ /×
9Wu“±ÏD\L»Ýý"ñ'ˆ´ÒnÕ×;‡t¾
É
Ë
ªÝßßç‰ÖÂ<�‹ªã÷C�×Ýy]_dŒ!¿!Á!½C]gŽk´[ßo_
t
{
¾
È
(Vy²·¼ÕûYó+Ýà.w€É–©¹âjöÜÖÕM ± 8"º ö<Î;ˆ-uèÝV	i	è	
¦
 gPè@èëÃç&=�ÁV^Œ«P]ø!$`Ñ[‚ÅîØEŒHœÂÕ8z`eŽ& Ç 2"2 1Õ‰à'Q•VÈ²äk k!B ¦›®U”ø	0	Š	l
â
"‚Ìü '1…þ8s�ädgu¨É×7D–´#S+L”µç,
Z b º ®!Þ"â"¯ Ä  6SØíoÖ:†×4½*Ø<		�
®
Ê
Œ©«ÜÞ]˜ˆqðKrÁ;äD€©q^cw ‹!�!¾!À![ öEB@þñ¶ýôvu\ h ""à"ä Ä1BDr„ÙBNœ¤¯ú¿!"Ðë÷�í;àUßf}¤lì@Ô[•NÒß3!¡"¡ %ºä/:ŠT

#
Ñ
oœWm
8×¾’ÃÇ`”§Æêó‹û€Úe;|‘ø ( ¶ Q"~"” £ ¬ '‡©ž�þAc÷µÏb	K�x|©Dl˜¨W³¼ÑgÅž�ð1m¬¸* ‡ Ä o!Z"û D³pùku`0
â;’Fš $„Ð#SÜâÿ×9;þÁßÉ+5Ä"- < B _ ¡ é ÷ %¦ºÜNRz›®ä/U”žø:CGaŠ–IT•äý	0	Š	ì	

#
l

Ñ
â
"8M‚Î¿Ìovœü '1W…�ôþ8gms�¤¨ÆäFd«3Bgu¨
28l‘É×éýYas~„¾×!7’ÃÇD`”œ§Æ<A–ê%ó‹´û#S·ð+L€”µÚçò,Ñþ
e…Úì;|‘	gø ( Z b ¶ º X!¡!®!Q"X"~"Þ"â"* 8 É û D "F³údp£€·ù
ku¡Ä`v3	§	ð	0
DS”» ×â;Yw•³Ñùû;’ÃFšî $„Ð#S�Üâÿ×@÷(9Yl;þÁßÉ+TV`bhj5µ‚"¹"Ä"c]ÒrØíÐÝÚ³ˆ…® BF�'±d%Îöß
ioéˆ,<ÉÛyÙ ›GÍ·û8™OËJ!Ë"~ 8_pxÒã²+‹á�Œ æ™êz¾	Á
õIÓôÄ<o{bh¹»¾À ø *!¸¼I…	 
At¡´L,Jh†¤ÂôÉ`cÿ�û=T[z’Ãz™ï÷Ap¯Ü‚’™¨«ÉÏÒØù`‹!"!V!ƒ!–!."x †K táó(6;oÛ
ãùOðŽHqRjˆøÛ"B ¶ c¦›®½ý"]U”ÒøåGry		$	0	\	Š	½	l
â
î
"‚Ìºü 
'1…ÊÕþ8s�Øä+dgu¨Í ~–Éíz×%7UDµÐÝï–�Úšª°o´á#Sz³+LVˆ”µç,bqx°Ý
¬%?IUan…Œ¤®êïœò Z b º ¿ ÷ !®!ý!œ"Þ"â"ì"ò")Í	¢êmÇåh^Á�­ÎÆGVq—Óí—¢Ù  Aª     ó  #        #XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬ÃÌ±Ï}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨5[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûü×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸ìS?Ç¹šwn�?®ƒéaìÁº02õèð´¾¤7ì½ÅÝwß3Nº(—àà÷d½Æï®ÂØ*Ÿ"½7xÃNÏ²ËCÃðËb€"�4îô¬8Ÿ‚Oèš†Ðî´ð½ÆR;/²ý$1‹<â4ôòú,a*$û ¢—$ó¶`pstìó8+wô‹§Â/R÷ß¤ûP7ß£çŒô:,¿ë !´X3‚Š¸´<“ø0cà��L¨=Ãíùûèá0ÏóÎnÈÔ£¤,ž‹DŽé1E9Y"}4é’èÔã5NûìS	ØMÇñSÍROÓ …ØñO …`ßs„0‹ a¸(ñF ³aálÔÂ0Œ$sú[Âx÷ž+a´`ª/ÂÅ;°7²{(SÀƒVKqB'c¨þ	,.Žoa†ª&	:ê�Ên¨Óúº;qg 88cö8­4ìÎÊ$bÊN ÷OwÆ@ß×p6^59®Î7}Æñ‚¢kOÙ3­“®=<\í»žÃ$%‹ß±˜Œ°•`�}$Î¶¢4ã³ÃòëEƒ8ë¥Š¨6ëØ Ôè"§×IðXõ8±:ö™ŸîD»ÌZÌsë‰…tª¾÷>¸˜WJ«ïsë‰…tª¾÷>¸˜WJ«ïsë‰…tª¾÷>¸˜WJ«ïN�½…Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       
_  ­  %  '  P     #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0!<?xml version="1.0"?><Features nextValidID="23"><Feature recentID="22" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5157-5186" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5187-5216" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5348-5862" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="luciferase" directionality="2" translationMW="60645.64" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3411-5063" color="#ffcc00" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2657-3245" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3246-3367" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="minP" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5106-5137" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5217-5246" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5247-5276" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5277-5306" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5307-5336" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3127" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="2744" maxFusedRunOn="2744" detectionMode="exactProteinMatch"><Segment range="3128-3139" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5060-5069" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1848-1850" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1851-1853" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1854-2567" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="1" translationMW="89.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2567" maxFusedRunOn="2567" detectionMode="exactProteinMatch"><Segment range="1842-1846" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7962" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7171-7962" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7963-8031" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6412-7000" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8032-8136" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6058-6064" color="#ffffff" type="standard"/><Segment range="6065-6082" color="#ffffff" type="standard"/><Segment name="-35" range="6083-6088" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6103-6124" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6034-6050" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1835" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="7:1:30">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  {<StrandColors><TopStrand><ColorRange range="5607..8962" colors="black"/><ColorRange range="5376..5606" colors="gray - 50"/><ColorRange range="5337..5375" colors="red"/><ColorRange range="5156..5336" colors="purple"/><ColorRange range="5069..5155" colors="blue"/><ColorRange range="3413..5068" colors="green"/><ColorRange range="3406..3412" colors="red"/><ColorRange range="3245..3405" colors="green"/><ColorRange range="2656..3244" colors="orange"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="0..185" colors="black"/></TopStrand><BottomStrand><ColorRange range="5607..8962" colors="black"/><ColorRange range="5376..5606" colors="gray - 50"/><ColorRange range="5337..5375" colors="red"/><ColorRange range="5156..5336" colors="purple"/><ColorRange range="5069..5155" colors="blue"/><ColorRange range="3413..5068" colors="green"/><ColorRange range="3406..3412" colors="red"/><ColorRange range="3245..3405" colors="green"/><ColorRange range="2656..3244" colors="orange"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="0..185" colors="black"/></BottomStrand></StrandColors>  @¢     ó  #        #ÿú«�VUº¦©¹ƒ²{™´à†h¡-W‘>Þ¾.¥Õ ylûœðslƒÃmðÏt#
83ælñÛ|`³Þ°;a3¢¬2`ƒCüƒÀŠ=»èØßkèÏ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒøc	ªï§6â0š·|Ù‚7»IÖã¸cÍ’”ôÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1T²Ïš©�¥‹a–X*é´ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS?YÍ_>ïó2ƒÆ¶–4àÜt*üx04„ÍsZX„¤íí¤7ý§z˜ëÃ²bˆ�h„Á—Z·Zïôî®3‹Ùa{Û´hbµ] å¡-ž–kùþÑbŠï^µâŸØTó‡K[{DþýAtÄ}Ô°c^5*hÜ	üsK›Ï
 ;ªhNÀE31tSL^
JÕ”7@¶­è�]¸`®äWŽ06àÛŠè?„ðã§Ý¾*Ù®8»¸¯—èn®æ»Jbj½�£Uƒ)éNN�WçB º¶õ8Ux«gE�WŠ¶tPÈUx«gE�WŠ¶tPÈUx«gE�WŠ¶tPË1k0G¹&e�NÆ¯NƒkÂx •ßS£ß`Q�Uv”P?Lö¹ýìQÀ|ðÄÓ�Õ=…3œþü7Û>§Ç7fÄ vŸœñ'¬F+æÎ`8YÕöÁ²�1N�+­�È #ø­°ˆ8ƒý9qÛ�q3ŽM€ÛOOß,šÈME@ãUír5QN|m�Tm¶Ø5}¯TØ�úÈzý¨÷ðë\IÇ¬�ÄN±ðWÛ?†Ø]$ƒIðhý@É?Ÿ7Íó¢/ÒØüÇýºÃ_FÛüwÎo´È#Ú·Ü:Ãb·Üà9èn0êÀ²;b|ëA9®ŒÓ£QæQ­n?ŽýÞjNºÎQ7»lu'”×£q=0À3ÎÓPBBòW:÷çÐÛÏ@ç‡EuôÏk†ßüPOƒ4�Ô �ë¬C” �èð”w€ˆ_LÃˆËÈ?Øm„Ãœ¥×üC�\îÛAÃƒ�ÓvÏžÄMë­²Ã…áµgP9ô$ÿO�oŽ5ßõÛ¬sÀ,ðøüqÆ<18üóK,ŽÜ%_añ²˜ƒÓÐ§_=E8Ì Š"²�•‡ÃR�ÏÍFó®ÚPõ5<‰e®D°<<ëGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô£ CÕÇ²á1G0Q4O4<ÀPP¼àh@ñ@rM¿3E½5ÝM=�PrÓ¯ñ×}Áh´�×Oþ/M051„¯<ƒY 1RüáÐ‚cý} Œ´ÕÝ#wÜ÷(øuÀ÷^ûeDt×ÐjgPAaSÙÀSY6Õ¥M2ovL•A5Ù)EŸK[«uj‰oD(j�WçB º¶¿þªàG••n©ªlð?0§¢tì½�!¿0·½4b3®Ê ºŽÐ0xí	 ¢ÀÓMÀ<³Æ "¸à’N78òû¢¡Jß¡�+®:,=†drˆ8¢J'½:#ý(òûèRÆ?­;Â·Ð"Ïì^·ì3è’�,¾…`Œï�É>°6…²8ëb “Þ7<�´-Ä@Ü:< �^´N�$ Û?³û<Î¢*rÏ/NÇÐ`7ßð…Ó¾Ïèíö2Ç0óµ:ì@äA‚÷ÿ °Óër‰½N1�.z„ Mˆ”/«Ó®BºK‚,0wˆ³Ç°”B}ð°½(®‚C�=»Ñë#�å$Dø”„è)%î‹bÐ-J�@0?Ù”nÙƒÙ€ùA!�•Ä0Bµ Aé'T‡ô -Tõ¿BÜb@‚à"ö0=U¡º_€†»
T#Ë³o¥BƒÚãDÑB_×.ë¡ÛŸ
!1¼ìvêô‡åW¥ —C“ð?f«GVtWt´tG]ÑqŽ4ÑÔs0Ì:Âý_5V´pCxªB».ùô_voï¹@7Õù\l©´,uWüRþË¿@£+Åì´¯}À¼ôgDä¤åä�ÐÍ]¤cÃuw7TtGGq\|àôéûðÀ °‚þ@.õnÂûÕ…Gÿ€ÒÖÖá¿Pm1/q¬ÕÓQ¦€YÀŸÀÇ4¹°AàÌñ! ¢º¦„ì4S3E1Åá ¤­YCtjÐ¾€7øÛ†
îExáón }¸®ƒøO Ð^:}Ñ›â ýšã‹»Šù~†ázîk´¦&«Øú5X2ž”äèõ~t «        ²  Û  Ý  U  £  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="23"><Feature recentID="22" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3778-3807" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-3777" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3102-3616" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3901-5553" color="#ffcc00" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5719-6307" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5597-5718" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3827-3858" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3718-3747" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3688-3717" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3658-3687" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3628-3657" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="Factor Xa site" directionality="1" translationMW="473.54" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5836" consecutiveTranslationNumbering="1" maxRunOn="6220" maxFusedRunOn="6220" detectionMode="exactProteinMatch"><Segment range="5825-5836" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3895-3904" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="2" translationMW="26942.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6397-7110" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="7111-7113" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="7114-7116" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6397" maxFusedRunOn="6397" detectionMode="exactProteinMatch"><Segment range="7118-7122" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1001" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="933-1001" color="#ccffcc" type="standard" translated="1"/><Segment range="1002-1793" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2552" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8447-8963" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="828-932" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="2876-2881" color="#ffffff" type="standard"/><Segment range="2882-2899" color="#ffffff" type="standard"/><Segment name="-10" range="2900-2906" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2840-2861" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2914-2930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7129-7148" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="7:0:59">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..3625" colors="red"/><ColorRange range="3626..3806" colors="purple"/><ColorRange range="3807..3893" colors="blue"/><ColorRange range="3894..5549" colors="green"/><ColorRange range="5550..5556" colors="red"/><ColorRange range="5557..5717" colors="green"/><ColorRange range="5718..6306" colors="orange"/><ColorRange range="6307..7084" colors="gray - 50"/><ColorRange range="7085..7116" colors="purple"/><ColorRange range="7117..7145" colors="red"/><ColorRange range="7146..8776" colors="gray - 50"/><ColorRange range="8777..8962" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..3625" colors="red"/><ColorRange range="3626..3806" colors="purple"/><ColorRange range="3807..3893" colors="blue"/><ColorRange range="3894..5549" colors="green"/><ColorRange range="5550..5556" colors="red"/><ColorRange range="5557..5717" colors="green"/><ColorRange range="5718..6306" colors="orange"/><ColorRange range="6307..7084" colors="gray - 50"/><ColorRange range="7085..7116" colors="purple"/><ColorRange range="7117..7145" colors="red"/><ColorRange range="7146..8776" colors="gray - 50"/><ColorRange range="8777..8962" colors="black"/></BottomStrand></StrandColors>  <     ì  "å        "åÿú«�VUº¦©¹ƒ²{™´à†h¡-W‘>Þ¾.¥Õ ylûœðslƒÃmðÏt#
83ælñÛ|`³Þ°;a3¢¬2`ƒCüƒÀŠ=»èØßkèÏ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒøc	ªï§6â0š·|Ù‚7»IÖã¸cÍ’”ôÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1T²Ïš©�¥‹a–X*é´ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS?YÍ_>ïó2ƒÆ¶–4àÜt*üx04„ÍsZX„¤íí¤7ý§z˜ëÃ²bˆ�h„Á—Z·Zïôî®3‹Ùa{Û´hbµ] å¡-ž–kùþÑbŠï^µâŸØTó‡K[{DþýAtÄ}Ô°c^5*hÜ	üsK›Ï
 ;ªhNÀE31tSL^
JÕ”7@¶­è�]¸`®äWŽ06àÛŠè?„ðã§Ý¾*Ù®8»¸¯—èn®æ»Jbj½�£Uƒ)éNN�WçB º¶õ8Ux«gE�WŠ¶tPÈUx«gE�WŠ¶tPÈUx«gE³³{‘fXìjôè6¼'Š	]õ:=öõWiEôÏkŸÞÅÏM8ÝSØS9Ïà�Ã}³ê|svlBiùÏzÄa2°àÑNaLæ…�_l ˆÓdèÒºØüŠ?ŠÛƒˆ?Ó—¹×18átØ´ôýòÉ¬„ÔT5Q¾×#UçÆÙÕFÛm�“QñçÐ:õM‰ß¬‡¯Ú�µÄœzÈüDëÕ}³øm…ÒH4Ÿ�Ô“ùó|ß:"ñ­-�ÌÛ¬5ôm¿Ç|æûL‚=«}Ã¬6+}Îž†ã¬#¶'Î±šèÍ:5eÖãøà�Ýæ¤ë¬å{¶ÇRqùMz7³Ó<í5$! õs¯~}¼ôpøtW_Lö¸mÿÅ qÔø3IBþºÄ9B�ß	Gx…ôÌ18Œ¼ƒý†ØL9Ê]Ä9ÕÎí±Ô88Ý0ÇlùàAÄÞºÛ |8Q>VuŸBOôñx6øã]ÿ]ºÇ<Ï�ÇcÃ�Ï4²Èà]ÂUöA yˆ==
uóÔSŒÂ¢+ qùPx|5(üÔo1í¥Q@ÃQ³È–ZäK ÃÃÎ±4vFv† u}×‘cHUV:šˆV¢æŽ¦¢_i”ãY]J00ýP¼{.1CsóDðÓCÌÎ„$ÑAËó4[Ðƒ]ÐÄÓØ-:ÿwÜ‹Hýtñ?âôÓPÐÃJóÈ5�S/ÁPN Ö?×Ò (ËM]Ò7}Ïr�‡\uï¶TGM}¦uV=œ5“a�PÚTÓ&÷dÉT]’”Yôµº·V¨–ôB†¨õ~t «kÿê®yYVêš¦Á¿ó
z'NËØñðÃq{ÓAö!³:ì ¨í€ŽÐ’
,4ÜË<`+Ž	 °Äãs�/±Š Áº­úòºã¢ÃØfG(ƒŠ$¢{Ó¢?Ðb�/¾…,cúÓ±Œ+},þÅë~Ã0.‰(òËèQFÎø“ë`ÁèQK#Ž¶ °	0áMãsÉ@‚Ü@€ôÀÓ£ÂÑ…ë@´èòB³û?³ÁÜáz ò§,òôì}}ÿ];ìþŽß`ƒ,s1kS®ÄD/ð>· 0È›Ôãòç¨@Â  ´Ðx‰Bú½1
ä+¤¸"Ã÷x‹<{	@„'ßÒŠè$8óÑ½²8RDO‰HN� â’^è¶-Ð©Ô #ý™Fí˜=˜ Ï‘8PlC ±q+PÀ0`ADÁ�"u@è@@ÕOPëô-Æ$ø./cÐ…Qú¥øa°¥B<»0öñÊT(=®4M%ýrîº¹ñà¢ÎÇn¯H~PµzP	t9?öj´ugEwKGDuÝãMG3Ã¬/ÕñSUkG0Š¤+²ïŸE÷fþû” #}Q/•ÆÀj�{BÀ÷UpÿÅ/à»ô
0ð"¼^ËJ÷ÜÏFtNJN^IÑÍÕÚF<7WsuGDtwÇÎNŸ¿ /à4ïVì _½XTñ-mnGûõÓ÷Á�]5êhœ	üsK›Ï
 ;ªhNÀE31tSL^
JÕ”7@¶­è�]¸`®äWŽ06àÛŠè?„ðã§Ý¾*Ù®8»¸¯—èn®æ»Jbj½�£Uƒ)éNN�WçB º¶        ”  ½  ¿  7  …  "ä  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,¬<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="19" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-3777" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3102-3616" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3871-5523" color="#ffcc00" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5689-6277" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5567-5688" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3797-3828" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3718-3747" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3688-3717" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3658-3687" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3628-3657" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="Factor Xa site" directionality="1" translationMW="473.54" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5806" consecutiveTranslationNumbering="1" maxRunOn="6190" maxFusedRunOn="6190" detectionMode="exactProteinMatch"><Segment range="5795-5806" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3865-3874" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="2" translationMW="26942.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6367-7080" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="7081-7083" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="7084-7086" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6367" maxFusedRunOn="6367" detectionMode="exactProteinMatch"><Segment range="7088-7092" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1001" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="933-1001" color="#ccffcc" type="standard" translated="1"/><Segment range="1002-1793" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2552" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8417-8933" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="828-932" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="2876-2881" color="#ffffff" type="standard"/><Segment range="2882-2899" color="#ffffff" type="standard"/><Segment name="-10" range="2900-2906" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2840-2861" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2914-2930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7099-7118" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:52:13">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  O<StrandColors><TopStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..6276" colors="blue"/><ColorRange range="6277..7054" colors="gray - 50"/><ColorRange range="7055..7086" colors="purple"/><ColorRange range="7087..7115" colors="red"/><ColorRange range="7116..8746" colors="gray - 50"/><ColorRange range="8747..8932" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..6276" colors="blue"/><ColorRange range="6277..7054" colors="gray - 50"/><ColorRange range="7055..7086" colors="purple"/><ColorRange range="7087..7115" colors="red"/><ColorRange range="7116..8746" colors="gray - 50"/><ColorRange range="8747..8932" colors="black"/></BottomStrand></StrandColors>  9¢     ä  "Ç        "Çÿú«�VUº¦©¹ƒ²{™´à†h¡-W‘>Þ¾.¥Õ ylûœðslƒÃmðÏt#
83ælñÛ|`³Þ°;a3¢¬2`ƒCüƒÀŠ=»èØßkèÏ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒøc	ªï§6â0š·|Ù‚7»IÖã¸cÍ’”ôÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1T²Ïš©�¥‹a–X*é´ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS?YÍ_>ïó2ƒÆ¶–4àÜt*üx04„ÍsZX„¤íí¤7ý§z˜ëÃ²bˆ�h„Á—Z·Zïôî®3‹Ùa{Û´hbµ] å¡-ž–kùþÑbŠï^µâŸØTó‡K[{DþýAtÄ}Ô°c^5*hÜ	üsK›Ï
 ;ªhNÀE31tSL^
JÕ”7@¶­è�]¸`®äWŽ06àÛŠè?„ðã§Ý¾*Ù®8»¸¯—èn®æ»Jbj½�£Uƒ)éNN�WçB º¶õ8Ux«gE�WŠ¶tPÈUx«gE�WŠ¶tPË1k0G¹&e�NÆ¯NƒkÂx •ßS£ß`Q�Uv”P?Lö¹ýìQÀ|ðÄÓ�Õ=…3œþü7Û>§Ç7fÄ vŸœñ'¬F+æÎ`8YÕöÁ²�1N�+­�È #ø­°ˆ8ƒý9qÛ�q3ŽM€ÛOOß,šÈME@ãUír5QN|m�Tm¶Ø5}¯TØ�úÈzý¨÷ðë\IÇ¬�ÄN±ðWÛ?†Ø]$ƒIðhý@É?Ÿ7Íó¢/ÒØüÇýºÃ_FÛüwÎo´È#Ú·Ü:Ãb·Üà9èn0êÀ²;b|ëA9®ŒÓ£QæQ­n?ŽýÞjNºÎQ7»lu'”×£q=0À3ÎÓPBBòW:÷çÐÛÏ@ç‡EuôÏk†ßüPOƒ4�Ô �ë¬C” �èð”w€ˆ_LÃˆËÈ?Øm„Ãœ¥×üC�\îÛAÃƒ�ÓvÏžÄMë­²Ã…áµgP9ô$ÿO�oŽ5ßõÛ¬sÀ,ðøüqÆ<18üóK,ŽÜ%_añ²˜ƒÓÐ§_=E8Ì Š"²�•‡ÃR�ÏÍFó®ÚPõ5<‰e®D°<<ëGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô£ CÕÇ²á1G0Q4O4<ÀPP¼àh@ñ@rM¿3E½5ÝM=�PrÓ¯ñ×}Áh´�×Oþ/M051„¯<ƒY 1RüáÐ‚cý} Œ´ÕÝ#wÜ÷(øuÀ÷^ûeDt×ÐjgPAaSÙÀSY6Õ¥M2ovL•A5Ù)EŸK[«uj‰oD(j�WçB º¶¿þªàG••n©ªlð?0§¢tì½�!¿0·½4b3®Ê ºŽÐ0xí	 ¢ÀÓMÀ<³Æ "¸à’N78òû¢¡Jß¡�+®:,=†drˆ8¢J'½:#ý(òûèRÆ?­;Â·Ð"Ïì^·ì3è’�,¾…`Œï�É>°6…²8ëb “Þ7<�´-Ä@Ü:< �^´N�$ Û?³û<Î¢*rÏ/NÇÐ`7ßð…Ó¾Ïèíö2Ç0óµ:ì@äA‚÷ÿ °Óër‰½N1�.z„ Mˆ”/«Ó®BºK‚,0wˆ³Ç°”B}ð°½(®‚C�=»Ñë#�å$Dø”„è)%î‹bÐ-J�@0?Ù”nÙƒÙ€ùA!�•Ä0Bµ Aé'T‡ô -Tõ¿BÜb@‚à"ö0=U¡º_€†»
T#Ë³o¥BƒÚãDÑB_×.ë¡ÛŸ
!1¼ìvêô‡åW¥ —C“ð?f«GVtWt´tG]ÑqŽ4ÑÔs0Ì:Âý_5V´pCxªB».ùô_voï¹@7Õù\l©´,uWüRþË¿@£+Åì´¯}À¼ôgDä¤åä�ÐÍ]¤cÃuw7TtGGq\|àôéûðÀ °‚þ@.õnÂûÕ…Gÿ€ÒÖÖá¿Pm1/q¬ÕÓQ¦€YÀŸÀÇ4¹°AàÌñ! ¢º¦„ì4S3E1Åá ¤­YCtjÐ¾€7øÛ†
îExáón }¸®ƒøO Ð^:}Ñ›â ýšã‹»Šù~†ázîk´¦&«Øú5X2ž”äèõ~t «        v  Ÿ  ¡    g  "Æ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *T<?xml version="1.0"?><Features recycledIDs="19" nextValidID="22"><Feature recentID="5" name="3' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3102-3616" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3841-5493" color="#ffcc00" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5659-6247" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5537-5658" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3767-3798" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3718-3747" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3688-3717" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3658-3687" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3628-3657" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="Factor Xa site" directionality="1" translationMW="473.54" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5776" consecutiveTranslationNumbering="1" maxRunOn="6160" maxFusedRunOn="6160" detectionMode="exactProteinMatch"><Segment range="5765-5776" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3835-3844" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="2" translationMW="26942.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6337-7050" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="7051-7053" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="7054-7056" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6337" maxFusedRunOn="6337" detectionMode="exactProteinMatch"><Segment range="7058-7062" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1001" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="933-1001" color="#ccffcc" type="standard" translated="1"/><Segment range="1002-1793" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2552" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8387-8903" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="828-932" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="2876-2881" color="#ffffff" type="standard"/><Segment range="2882-2899" color="#ffffff" type="standard"/><Segment name="-10" range="2900-2906" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2840-2861" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2914-2930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7069-7088" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:52:9">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  O<StrandColors><TopStrand><ColorRange range="8717..8902" colors="black"/><ColorRange range="7086..8716" colors="gray - 50"/><ColorRange range="7057..7085" colors="red"/><ColorRange range="7025..7056" colors="purple"/><ColorRange range="6247..7024" colors="gray - 50"/><ColorRange range="3587..6246" colors="blue"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="0..3355" colors="black"/></TopStrand><BottomStrand><ColorRange range="8717..8902" colors="black"/><ColorRange range="7086..8716" colors="gray - 50"/><ColorRange range="7057..7085" colors="red"/><ColorRange range="7025..7056" colors="purple"/><ColorRange range="6247..7024" colors="gray - 50"/><ColorRange range="3587..6246" colors="blue"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="0..3355" colors="black"/></BottomStrand></StrandColors>  :r   
  ä  "Ç        "ÇXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬ÃÌ±Ï}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨5[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûü×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸ìS?Ç¹šwn�?®ƒéaìÁº02õèð´¾¤7ì½ÅÝwß3Nº(—àà÷d½Æï®ÂØ*Ÿ"½7xÃNÏ²ËCÃðËb€"�4îô¬8Ÿ‚Oèš†Ðî´ð½ÆR;/²ý$1‹<â4ôòú,a*$û ¢—$ó¶`pstìó8+wô‹§Â/R÷ß¤ûP7ß£çŒô:,¿ë !´X3‚Š¸´<“ø0cà��L¨=Ãíùûèá0ÏóÎnÈÔ£¤,ž‹DŽé1E9Y"}4é’èÔã5NûìS	ØMÇñSÍROÓ …ØñO …`ßs„0‹ a¸(ñF ³aálÔÂ0Œ$sú[Âx÷ž+a´`ª/ÂÅ;°7²{(SÀƒVKqB'c¨þ	,.Žoa†ª&	:ê�Ên¨Óúº;qg 88cö8­4ìÎÊ$bÊN ÷OwÆ@ß×p6^59®Î7}Æñ‚¢kOÙ3­“®=<\í»žÃ$%‹ß±˜Œ°•`�}$Î¶¢4ã³ÃòëEƒ8ë¥Š¨6ëØ Ôè"§×IðXõ8±:ö™ŸîD»ÌZÌsë‰…tª¾÷>¸˜WJ«ïsë‰…tª¾÷>¸˜WJ«ïN�½…Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       
_  ­  %  '  P  Ä  "Æ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +(<?xml version="1.0"?><Features recycledIDs="19" nextValidID="22"><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5288-5802" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="luciferase" directionality="2" translationMW="60645.64" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3411-5063" color="#ffcc00" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2657-3245" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3246-3367" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="minP" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5106-5137" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5157-5186" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5187-5216" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5217-5246" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5247-5276" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3127" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="2744" maxFusedRunOn="2744" detectionMode="exactProteinMatch"><Segment range="3128-3139" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5060-5069" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1848-1850" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1851-1853" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1854-2567" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="1" translationMW="89.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2567" maxFusedRunOn="2567" detectionMode="exactProteinMatch"><Segment range="1842-1846" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7902" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7111-7902" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7903-7971" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6352-6940" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7972-8076" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5998-6004" color="#ffffff" type="standard"/><Segment range="6005-6022" color="#ffffff" type="standard"/><Segment name="-35" range="6023-6028" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6043-6064" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5974-5990" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1835" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:48:2">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  K<StrandColors><TopStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="2656..5315" colors="blue"/><ColorRange range="5316..5546" colors="gray - 50"/><ColorRange range="5547..8902" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="2656..5315" colors="blue"/><ColorRange range="5316..5546" colors="gray - 50"/><ColorRange range="5547..8902" colors="black"/></BottomStrand></StrandColors>  &Ï   	  ä  "Ç        "ÇXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬ÃÌ±Ï}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨5[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûü×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸ìS?Ç¹šwn�?®ƒéaìÁº02õèð´¾¤7ì½ÅÝwß3Nº(—àà÷d½Æï®ÂØ*Ÿ"½7xÃNÏ²ËCÃðËb€"�4îô¬8Ÿ‚Oèš†Ðî´ð½ÆR;/²ý$1‹<â4ôòú,a*$û ¢—$ó¶`pstìó8+wô‹§Â/R÷ß¤ûP7ß£çŒô:,¿ë !´X3‚Š¸´<“ø0cà��L¨=Ãíùûèá0ÏóÎnÈÔ£¤,ž‹DŽé1E9Y"}4é’èÔã5NûìS	ØMÇñSÍROÓ …ØñO …`ßs„0‹ a¸(ñF ³aálÔÂ0Œ$sú[Âx÷ž+a´`ª/ÂÅ;°7²{(SÀƒVKqB'c¨þ	,.Žoa†ª&	:ê�Ên¨Óúº;qg 88cö8­4ìÎÊ$bÊN ÷OwÆ@ß×p6^59®Î7}Æñ‚¢kOÙ3­“®=<\í»žÃ$%‹ß±˜Œ°•`�}$Î¶¢4ã³ÃòëEƒ8ë¥Š¨6ëØ Ôè"§×IðXõ8±:ö™ŸîD»ÌZÌsë‰…tª¾÷>¸˜WJ«ïsë‰…tª¾÷>¸˜WJ«ïN�½…Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       
_  ­  %  '  P  Ä  "Æ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  …<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2657-5316" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1848-1850" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1851-1853" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1854-2567" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="1" translationMW="89.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2567" maxFusedRunOn="2567" detectionMode="exactProteinMatch"><Segment range="1842-1846" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7902" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7111-7902" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7903-7971" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6352-6940" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2628-5802" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7972-8076" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5998-6004" color="#ffffff" type="standard"/><Segment range="6005-6022" color="#ffffff" type="standard"/><Segment name="-35" range="6023-6028" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6043-6064" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5974-5990" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1835" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:44:1">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  K<StrandColors><TopStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="2656..5315" colors="blue"/><ColorRange range="5316..5546" colors="gray - 50"/><ColorRange range="5547..8902" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2655" colors="gray - 50"/><ColorRange range="2656..5315" colors="blue"/><ColorRange range="5316..5546" colors="gray - 50"/><ColorRange range="5547..8902" colors="black"/></BottomStrand></StrandColors>  -     +  c         cXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬ÃÌ±Ï}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@   
1      
(®ZTR

BASE      
È"  xÁ11 ÄÀ§¶ra&¥8á¯Ÿùm¹Í²i¥lÒ¤6ÛV%›²tÛk#çÖœ›Å\lZÓ�9ç˜Ì­¥Â6¦k‘i›Ì‚Pb*lnÌ¥Ì% “YÑœ,æ²‚©	°é ·å€L¨Un´Á³�"¤ÈBDŠ$ '±µmN`l 1Ba�kŽÄP`+`k›%YÉ·0!¹ Ô©Ä\¹r$0U£b•NÎU:�ÀÔÁÀ ‚:m“&V˜‘•‰Y™ ,Ú²!1²Ñ¬IM"[¤è Ót#‡³Ð�
²©s0uª‚U™rm[€«éˆJ“@ÍéÔµL‹´éÎÚ6fB`äV•Th¥ÜDŒ2’d²°È,#2rdVd«ŒÜ¶–
¨4!k0j³rPÓé6—áDcƒÚ\Æææ–Zƒ"‰dn)lPmºÄ9”Aµ�s‹ÓÚ\lUR@–Žs0 ±0©›PJ•€€ÔØ*Ú„@¢ X €Pn„…ÂÕ‚	ÄŠhŠ ¬¥°–sQk©2 (ÛF9 ÇrlÎmL€ˆ`“5¦D4j“áf¤s)Ê„›éÀ�¦b@œ¡8ÆÌÔ@pæhsƒæ!é6Ô3Hr0 ±ÍæP2)]cÃÜ” j:XM œ›”[!
b8„ÁÒœl*N'YTÓ¤á$ŠÃ¶¡06‹‰(NÅ©¸„ACÙ$äf±He“L „9�ƒ†b2“b‚4 c¹Éd8Šb3–€›Ò0˜Nd°(ŠM‡CÙhlÁinNÅ1 Ídá$7•…£Øt:”�©26Igc£±1al­ÅRpl277”Å¦¹Ql.ÆÆÂéP&&“Œ“¹1 ŠCL’Œ90�œls ÎMÄÅX$é,Ì� ¨‹¡,N²±Ù¢ê×¶µm®jÕŒ`nÛTuç}çœïÜ{ï¹÷}ï|ß{ïûî9[léjl[ensåH[Q-K‚ª!-Ó5$©¶„ÊKZI¶”ŠUek[�–dDµœÎ2,cÊ†)¦™ŒÌJrÈÀHK••0dL‚
ÔQ! ­„¡èF,!¡$IÇT	%KeäØ
L³ÕMrT°”$„ÌéŠDÔaP$
b&:((@’%…(£
�˜Ãº¨T�sî{ß{ï¼÷ÝóÝ÷Î;ç}ß{çžsÞ9ç»ç¼óÝóî¹çœ÷Þ=÷œóÎ9ß=ï½÷Þ»ç¾ïž÷î;ß{ßû¾÷Þ÷î=çÜwÏ»ç¼ï�wßÛ»ç}ï¾ï¾û�óÝï»÷ûîýî½÷;÷Ýw¾sÞ[�¡¸(A (…Úrn¢‹ƒ�4fPM„Ápcl"“¢-Û %…±f0\Eh,­˜“áp$ŠÊ$ea*SP€b¸¹,Õ,™L—D0%U¤M l•‹)QLÐ`¸ÜÜLÂ ˜Mq‚d2a‚A.çÄÊ&„jY6Ab¹Xª0tÈ˜„ ’cÀÌ¨�f°5!ÜÜTƒ‰0¥(TÍÅÜR$-+2¦`HÑ%¤MÓM¬†B%9Q$ÅE˜l–Ô4l”árÎ$jâæ&mkf*ƒÉTæÊ-jÍ(Ù$‘Í­I„›hc9p› s±Ù&¦¹±7Á4·¥†ätM4Cu¹HKps©†¨,Gn¡cl¢‰&ÚT¥²ÚH�,–‹©¸´Ê 7‰Pn†�°Ü5e[êf7ÍÆ’J7�´%-X.—‹*[.´IQ%Ãrº¨’	T”Äpk84•”\K²˜LB$,›Ë°&©Â˜0.7¦2`c¨,77’MZ’ŒÁp�K+[1�5‘bMŠ„	nH,ÂÍF(6mË`®ebhÛ—&¨¦b)–R™L’0
gjsK‘T ¨-«RKS6)Íj°1è–°Iº-œ¬ÖÍ�¸âŠl¹�YC(²å6fU¡È–Û˜UA0„"[ncVÁ¤tÁr›dÓJÙ¤Im¶!¬J6eé¶5ÖFÎ­97‹¹Ø´¦sÎ1™[K…mL×"Ó6™	 ÄTØÜ˜K˜K4@ &³¢97XÌeS`ÓAnË™P«Ühƒ-f!EH‘…ˆI NbkÛœÀØ b„"Â×‰! 6ÀlÁÖ6 JfÉG™FH”:5‚È™+GŒ‰ªQ±Ô6fE¹Y:76VäÔ¦3ÜZm¶9b«	#«Ü‚±F°5‚Ü,\:‹ÕÒ1‘¹ÍIªs+s[Y$ÆV0ÆÆNÐMÖ6³mBª	m9sÒFŒÕVb‰ÄH@*˜¡nƒ&ÉjË-6DTç­ÄV*R#GH"¡a¡$Šm1‰Ûmkh-s‹©®™nsc™“mS˜l›ÂtÛ¶)ˆ%)VkJ  äp²Û¶pHlÆœ¦¸pLGk›k0Å
€t“€0‘²À	R]ƒ„Š€BÝÄ©ˆ5‰!Ù´EÌB³iXH°I*™Ép ‹	8�D¨ÚF£œÁh›c8j!Œ”É„‘¶1]MæÄiË¦hÒ˜³(7ÓÚh(�ÑÜÜ˜­Ò‰D’›0kPè¶ÍYÚ$ÊÊ6¶Á@Ò&šVUV²Éd®†ÈÜ¶mºˆÍÍ•‹*ª‘Cb¦n1¬M]•Ær®Û6]ƒæ‚ªmµmËÍªm±EÛl­ål¥‘cÖ`Kj‹™S-bâ"£UX,X³„fsm4\C�J)ÀL’-×@œ†Ä@	æªÅdkL˜ ›
Ndn¦A²Q-‚3Zme	ê&ÍÙf³fmÍJdÃ²„•4¦,L3†„a¥-¥X1	´€›â¬±\ ,ËdÛ6Ñš…�Ë˜›m–‚Ì¬Åˆ¥µ”ÉHÖVD�Œ%,°Í%UdŽ4g#6ØŒ9·f¶Dqà,‰PŒ­¡®­�cMæFµm&±Xé¶aQ¨â¶±é¢’å\0—mÀ“¹(&‹ªQ1˜X‘š“mÓMÙ¶Ù¶MÝÚTC·e¹5b�(¨Š­ÕÊMV-¦ÖÊˆåD×X«Ü²UsÛÖ6¶5Õ‘±
Àm3a�Ãhs¬V‹åXmÐÚ¶5ÅÃ)Pa„ˆšË¡¹m9Æ’I€Î¡1&ed›èÔÉ›€11&j–¢f¨’R-Qs2W6rÎd°–hæ¶2æÛtn[YLV+¦$²‘¥n*.4Q!
i5„IŠÛT]&É¦¸Ú,µaj0c8,Û6Šlº¥É’MçR6f (UH¬Cp›H,'àfŠpQ Íe(®5E˜l ƒL4— 0\b Øœâj[emsL×b¦Á”hV9bck1˜ä&´æl8aÍµmm›«Z5#˜Û6UýDcÓ   h<AlignableSequences><Sequence name="MGCV Bidirection Vector" ID="0" sortOrder="0"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  D<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1848-1850" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1851-1853" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1854-2567" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="1" translationMW="89.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2567" maxFusedRunOn="2567" detectionMode="exactProteinMatch"><Segment range="1842-1846" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5242" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4451-5242" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5243-5311" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3692-4280" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2628-3142" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5312-5416" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3338-3344" color="#ffffff" type="standard"/><Segment range="3345-3362" color="#ffffff" type="standard"/><Segment name="-35" range="3363-3368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3383-3404" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3314-3330" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1835" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:43:14">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ‰<StrandColors><TopStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2886" colors="gray - 50"/><ColorRange range="2887..6242" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1877" colors="purple"/><ColorRange range="1878..2886" colors="gray - 50"/><ColorRange range="2887..6242" colors="black"/></BottomStrand></StrandColors>   \<?xml version="1.0"?><SequenceCaseChanges><ToLower ranges="0..6242"/></SequenceCaseChanges>
  -ž     ;  ¢         ¢XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬ÃÌ±Ïyzïâ…u4‹E¿ß=öM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R_0?¼eYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾½±�+i�Ó4TN E¢¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pÍA`��A3õÒÿÃÜãßg÷<WS;a?ƒñºÿl!‹fC=5ó~ ÏcÃÜc/‘i/ZŠTt´‚ô;’¨{]fßNù‘'ÙVU¦¤»ÑU    
1      
(®ZTR

BASE      
È"  xÁ11 ÄÀ§¶ra&¥8á¯Ÿùm¹Í²i¥lÒ¤6ÛV%›²tÛk#çÖœ›Å\lZÓ�9ç˜Ì­¥Â6¦k‘i›Ì‚Pb*lnÌ¥Ì% “YÑœ,æ²‚©	°é ·å€L¨Un´Á³�"¤ÈBDŠ$ '±µmN`l 1Ba�kŽÄP`+`k›%YÉ·0!¹ Ô©Ä\¹r$0U£b•NÎU:�ÀÔÁÀ ‚:m“&V˜‘•‰Y™ ,Ú²!1²Ñ¬IM"[¤è Ót#‡³Ð�
²©s0uª‚U™rm[€«éˆJ“@ÍéÔµL‹´éÎÚ6fB`äV•Th¥ÜDŒ2’d²°È,#2rdVd«ŒÜ¶–
¨4!k0j³rPÓé6—áDcƒÚ\Æææ–Zƒ"‰dn)lPmºÄ9”Aµ�s‹ÓÚ\lUR@–Žs0 ±0©›PJ•€€ÔØ*Ú„@¢ X €Pn„…ÂÕ‚	ÄŠhŠ ¬¥°–sQk©2 (ÛF9 ÇrlÎmL€ˆ`“5¦D4j“áf¤s)Ê„›éÀ�¦b@œ¡8ÆÌÔ@pæhsƒæ!é6Ô3Hr0 ±ÍæP2)]cÃÜ” j:XM œ›”[!
b8„ÁÒœl*N'YTÓ¤á$ŠÃ¶¡06‹‰(NÅ©¸„ACÙ$äf±He“L „9�ƒ†b2“b‚4 c¹Éd8Šb3–€›Ò0˜Nd°(ŠM‡CÙhlÁinNÅ1 Ídá$7•…£Øt:”�©26Igc£±1al­ÅRpl277”Å¦¹Ql.ÆÆÂéP&&“Œ“¹1 ŠCL’Œ90�œls ÎMÄÅX$é,Ì� ¨‹¡,N²±Ù¢ê×¶µm®jÕŒ`nÛTuç}çœïÜ{ï¹÷}ï|ß{ïûî9[léjl[ensåH[Q-K‚ª!-Ó5$©¶„ÊKZI¶”ŠUek[�–dDµœÎ2,cÊ†)¦™ŒÌJrÈÀHK••0dL‚
ÔQ! ­„¡èF,!¡$IÇT	%KeäØ
L³ÕMrT°”$„ÌéŠDÔaP$
b&:((@’%…(£
�˜Ãº¨T�sî{ß{ï¼÷ÝóÝ÷Î;ç}ß{çžsÞ9ç»ç¼óÝóî¹çœ÷Þ=÷œóÎ9ß=ï½÷Þ»ç¾ïž÷î;ß{ßû¾÷Þ÷î=çÜwÏ»ç¼ï�wßÛ»ç}ï¾ï¾û�óÝï»÷ûîýî½÷;÷Ýw¾sÞ[�¡¸(A (…Úrn¢‹ƒ�4fPM„Ápcl"“¢-Û %…±f0\Eh,­˜“áp$ŠÊ$ea*SP€b¸¹,Õ,™L—D0%U¤M l•‹)QLÐ`¸ÜÜLÂ ˜Mq‚d2a‚A.çÄÊ&„jY6Ab¹Xª0tÈ˜„ ’cÀÌ¨�f°5!ÜÜTƒ‰0¥(TÍÅÜR$-+2¦`HÑ%¤MÓM¬†B%9Q$ÅE˜l–Ô4l”árÎ$jâæ&mkf*ƒÉTæÊ-jÍ(Ù$‘Í­I„›hc9p› s±Ù&¦¹±7Á4·¥†ätM4Cu¹HKps©†¨,Gn¡cl¢‰&ÚT¥²ÚH�,–‹©¸´Ê 7‰Pn†�°Ü5e[êf7ÍÆ’J7�´%-X.—‹*[.´IQ%Ãrº¨’	T”Äpk84•”\K²˜LB$,›Ë°&©Â˜0.7¦2`c¨,77’MZ’ŒÁp�K+[1�5‘bMŠ„	nH,ÂÍF(6mË`®ebhÛ—&¨¦b)–R™L’0
gjsK‘T ¨-«RKS6)Íj°1è–°Iº-œ¬ÖÍ�¸âŠl¹�YC(²å6fU¡È–Û˜UA0„"[ncVÁ¤tÁr›dÓJÙ¤Im¶!¬J6eé¶5ÖFÎ­97‹¹Ø´¦sÎ1™[K…mL×"Ó6™	 ÄTØÜ˜K˜K4@ &³¢97XÌeS`ÓAnË™P«Ühƒ-f!EH‘…ˆI NbkÛœÀØ b„"Â×‰! 6ÀlÁÖ6 JfÉG™FH”:5‚È™+GŒ‰ªQ±Ô6fE¹Y:76VäÔ¦3ÜZm¶9b«	#«Ü‚±F°5‚Ü,\:‹ÕÒ1‘¹ÍIªs+s[Y$ÆV0ÆÆNÐMÖ6³mBª	m9sÒFŒÕVb‰ÄH@*˜¡nƒ&ÉjË-6DTç­ÄV*R#GH"¡a¡$Šm1‰Ûmkh-s‹©®™nsc™“mS˜l›ÂtÛ¶)ˆ%)VkJ  äp²Û¶pHlÆœ¦¸pLGk›k0Å
€t“€0‘²À	R]ƒ„Š€BÝÄ©ˆ5‰!Ù´EÌB³iXH°I*™Ép ‹	8�D¨ÚF£œÁh›c8j!Œ”É„‘¶1]MæÄiË¦hÒ˜³(7ÓÚh(�ÑÜÜ˜­Ò‰D’›0kPè¶ÍYÚ$ÊÊ6¶Á@Ò&šVUV²Éd®†ÈÜ¶mºˆÍÍ•‹*ª‘Cb¦n1¬M]•Ær®Û6]ƒæ‚ªmµmËÍªm±EÛl­ål¥‘cÖ`Kj‹™S-bâ"£UX,X³„fsm4\C�J)ÀL’-×@œ†Ä@	æªÅdkL˜ ›
Ndn¦A²Q-‚3Zme	ê&ÍÙf³fmÍJdÃ²„•4¦,L3†„a¥-¥X1	´€›â¬±\ ,ËdÛ6Ñš…�Ë˜›m–‚Ì¬Åˆ¥µ”ÉHÖVD�Œ%,°Í%UdŽ4g#6ØŒ9·f¶Dqà,‰PŒ­¡®­�cMæFµm&±Xé¶aQ¨â¶±é¢’å\0—mÀ“¹(&‹ªQ1˜X‘š“mÓMÙ¶Ù¶MÝÚTC·e¹5b�(¨Š­ÕÊMV-¦ÖÊˆåD×X«Ü²UsÛÖ6¶5Õ‘±
Àm3a�Ãhs¬V‹åXmÐÚ¶5ÅÃ)Pa„ˆšË¡¹m9Æ’I€Î¡1&ed›èÔÉ›€11&j–¢f¨’R-Qs2W6rÎd°–hæ¶2æÛtn[YLV+¦$²‘¥n*.4Q!
i5„IŠÛT]&É¦¸Ú,µaj0c8,Û6Šlº¥É’MçR6f (UH¬Cp›H,'àfŠpQ Íe(®5E˜l ƒL4— 0\b Øœâj[emsL×b¦Á”hV9bck1˜ä&´æl8aÍµmm›«Z5#˜Û6UýDcÓ   h<AlignableSequences><Sequence name="MGCV Bidirection Vector" ID="0" sortOrder="0"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  \<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1911-1913" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1914-1916" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1917-2630" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="P2A" directionality="1" translationMW="1987.23" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1895" consecutiveTranslationNumbering="1" maxRunOn="2630" maxFusedRunOn="2630" detectionMode="exactProteinMatch"><Segment range="1842-1898" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5305" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4514-5305" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5306-5374" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3755-4343" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2691-3205" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5375-5479" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3401-3407" color="#ffffff" type="standard"/><Segment range="3408-3425" color="#ffffff" type="standard"/><Segment name="-35" range="3426-3431" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3446-3467" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3377-3393" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1835" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="6:42:14">2025.10.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ç<StrandColors><TopStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1908" colors="black"/><ColorRange range="1909..1940" colors="purple"/><ColorRange range="1941..2718" colors="gray - 50"/><ColorRange range="2719..6305" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..185" colors="black"/><ColorRange range="186..1816" colors="gray - 50"/><ColorRange range="1817..1845" colors="red"/><ColorRange range="1846..1908" colors="black"/><ColorRange range="1909..1940" colors="purple"/><ColorRange range="1941..2718" colors="gray - 50"/><ColorRange range="2719..6305" colors="black"/></BottomStrand></StrandColors>   \<?xml version="1.0"?><SequenceCaseChanges><ToLower ranges="0..6305"/></SequenceCaseChanges>
  !è     T           XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬äÊ•éN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü±Ïyzïâ…u4‹E¿ß=öM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R_0?¼eYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾½±�+i�Ó4TN E¢¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pÍA`��A3õÒÿÃÜãßg÷<WS;a?ƒñºÿl!‹fC=5ó~ ÏcÃÜc/‘i/ZŠTt´‚ô;’¨{]fßNù‘'ÙVU¦¤»ÑU    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1828-1935" color="#ccffcc" type="standard"/></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1827" color="#ccffcc" type="standard"/></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3477-3493" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3546-3567" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3501-3507" color="#ffffff" type="standard"/><Segment range="3508-3525" color="#ffffff" type="standard"/><Segment name="-35" range="3526-3531" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5475-5579" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2791-3305" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3855-4443" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5405" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4614-5405" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5406-5474" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="P2A" directionality="1" translationMW="1987.23" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1995" consecutiveTranslationNumbering="1" maxRunOn="2730" maxFusedRunOn="2730" detectionMode="exactProteinMatch"><Segment range="1942-1998" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2011-2013" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2014-2016" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2017-2730" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>f473a3e5-3b02-458e-9655-d98de14c7edf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="1:53:57">2024.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c<StrandColors><TopStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6405" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6405" colors="black"/></BottomStrand></StrandColors>  !Î     :             XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬Ë÷—®þ(WS@±D[ð=ð³ß`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%óðÆU•F©©.ôU@aQÿ~$*NN”—=ª5�Vv^dE+FÔ)ÑÓE˜WA›ˆ%4¯�ðíÔ]/ôcóûM*äEöÑŠ½?Ô…aþ#é¨^_[JÎ»:â³3®;üNÖU÷_û{ÏKï2ÏÀŸÈ›ÖWû£J6ñè‚ìâ¾€@»î-0êØ	t«¡J]v¸@�KiIo‡µ¤÷Š¡ov{Ö{á[Û¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1828-1833" color="#ccffcc" type="standard"/></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1827" color="#ccffcc" type="standard"/></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3444-3465" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3399-3405" color="#ffffff" type="standard"/><Segment range="3406-3423" color="#ffffff" type="standard"/><Segment name="-35" range="3424-3429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5373-5477" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2689-3203" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3753-4341" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5303" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4512-5303" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5304-5372" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="P2A" directionality="1" translationMW="1987.23" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1893" consecutiveTranslationNumbering="1" maxRunOn="2628" maxFusedRunOn="2628" detectionMode="exactProteinMatch"><Segment range="1840-1896" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1909-1911" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1912-1914" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1915-2628" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>f473a3e5-3b02-458e-9655-d98de14c7edf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="1:53:27">2024.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c<StrandColors><TopStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6303" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6303" colors="black"/></BottomStrand></StrandColors>  !     9  š         šXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN…¬±Ïyzïâ…u4‹E¿ß=öM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R_0?¼eYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾½±�+i�Ó4TN E¢¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pÍA`��A3õÒÿÃÜãßg÷<WS;a?ƒñºÿl!‹fC=5ó~ ÏcÃÜc/‘i/ZŠTt´‚ô;’¨{]fßNù‘'ÙVU¦¤»ÑU    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  \<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1827" color="#ccffcc" type="standard"/></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3385" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3438-3459" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3393-3399" color="#ffffff" type="standard"/><Segment range="3400-3417" color="#ffffff" type="standard"/><Segment name="-35" range="3418-3423" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5367-5471" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2683-3197" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3747-4335" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5297" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4506-5297" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5298-5366" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="P2A" directionality="1" translationMW="1987.23" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1887" consecutiveTranslationNumbering="1" maxRunOn="2622" maxFusedRunOn="2622" detectionMode="exactProteinMatch"><Segment range="1834-1890" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="1903-1905" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1906-1908" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1909-2622" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>f473a3e5-3b02-458e-9655-d98de14c7edf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="1:52:39">2024.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c<StrandColors><TopStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6297" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1820" colors="black"/><ColorRange range="1821..1826" colors="blue"/><ColorRange range="1827..6297" colors="black"/></BottomStrand></StrandColors>  #'     �  +         +XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN�}€‹ùý;à;ãˆ|sÛŒvôaýøôø¹ôøGH}½"ÐôÄ¹îƒ ›Mü5ûN8npÏL~42>MÓ0ïý'O…}64|AÄ‰ë�MCáùþEúÁ7Ôà^ÁHÁàÃ;…#N4ÒàÎ‚ðø7“!N/�B/wÛ~¹çczÈSD=@øÃE±þq}IÓJÔ]CßáóÇ}4×|ùøS‹“áûÄóßž¨Ûœ0çž±ç€=!‚ß"ôÛ¶'ÿïçàuÄE5Ã}¶÷mQTßˆ÷DÓýN+€þ ƒÃ#Šâœ>8-Òâ2�õÓEÑ=vaÿÑCÐø}?ŸOç?ãÑ³Ï�Ñ=JÓFóÄtó×ãA¿_N'\Nà4pYÇ#�TQÐñ54ÑQ_í¹×?�FùÓ°ßP1E Ôqƒâ}â÷Ï}AßœsøÝ�àÿÑ,sÞ^»ø¡]M bÅoÀ÷ÂÏ}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Æ<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1831" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4794-4810" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4863-4884" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4818-4824" color="#ffffff" type="standard"/><Segment range="4825-4842" color="#ffffff" type="standard"/><Segment name="-35" range="4843-4848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6792-6896" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4108-4622" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5172-5760" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6722" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5931-6722" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6723-6791" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="P2A" directionality="1" translationMW="1987.23" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3312" consecutiveTranslationNumbering="1" maxRunOn="4047" maxFusedRunOn="4047" detectionMode="exactProteinMatch"><Segment range="3259-3315" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3328-3330" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3331-3333" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3334-4047" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>f473a3e5-3b02-458e-9655-d98de14c7edf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="1:48:1">2024.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..7722" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..7722" colors="black"/></BottomStrand></StrandColors>  $O         7          7XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN�}€‹ùý;à;ãˆ|sÛŒvôaýøôø¹ôøGH}½"ÐôÄ¹îƒ ›Mü5ûN8npÏL~42>MÓ0ïý'O…}64|AÄ‰ë�MCáùþEúÁ7Ôà^ÁHÁàÃ;…#N4ÒàÎ‚ðø7“!N/�B/wÛ~¹çczÈSD=@øÃE±þq}IÓJÔ]CßáóÇ}4×|ùøS‹“áûÄóßž¨Ûœ0çž±ç€=!‚ß"ôÛ¶'ÿïçàuÄE5Ã}¶÷mQTßˆ÷DÓýN+€þ ƒÃ#Šâœ>8-Òâ2�õÓEÑ=vaÿÑCÐø}?ŸOç?ãÑ³Ï�Ñ=JÓFóÄtó×ãA¿_N'\Nà4pYÇ#�TQÐñ54ÑQ_í¹×?�FùÓ°ßP1E Ôqƒâ}â÷Ï}AßœsøÝ�àÿÑ,}�½kÏÿÿåÊx<SÎ�eŒ¨¹˜¦«ßf�.¨5ˆ@üø?‹®‡käêÿ»=PXÔ.(X²Ò¾àS®…]râL¨ÐÓÿ÷àÇB>ãU=È™FwãT0ÝÒ=Ê!((�Q-X;¾ÔÉ­u áA�ÑBÚ&¸n ûÝŽ§b*K¥Urä?ü_p ªú…Wq†YƒÝ}‚P1â·À �Ûà±81É\=Ô­2.@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô1zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—ÌÀoVU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…ol`JÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7246" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6455-7246" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7247-7315" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5696-6284" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4632-5146" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7316-7420" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5342-5348" color="#ffffff" type="standard"/><Segment range="5349-5366" color="#ffffff" type="standard"/><Segment name="-35" range="5367-5372" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5387-5408" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5318-5334" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1831" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26914.03" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3852-3854" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3855-3857" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3858-4571" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="IRES" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3288-3840" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ¹<?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Primer 1" sequence="GTGGTACCTCACCCTTACCG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite><BindingSite simplified="1" location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>fa2a8599-04f1-4ed3-adb0-7ad0f329b615</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="5:14:42">2022.6.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ³<StrandColors><TopStrand><ColorRange range="3258..4570" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="3258..4570" colors="magenta"/></BottomStrand></StrandColors>  $P     !   :          :XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN�}€‹ùý;à;ãˆ|sÛŒvôaýøôø¹ôøGH}½"ÐôÄ¹îƒ ›Mü5ûN8npÏL~42>MÓ0ïý'O…}64|AÄ‰ë�MCáùþEúÁ7Ôà^ÁHÁàÃ;…#N4ÒàÎ‚ðø7“!N/�B/wÛ~¹çczÈSD=@øÃE±þq}IÓJÔ]CßáóÇ}4×|ùøS‹“áûÄóßž¨Ûœ0çž±ç€=!‚ß"ôÛ¶'ÿïçàuÄE5Ã}¶÷mQTßˆ÷DÓýN+€þ ƒÃ#Šâœ>8-Òâ2�õÓEÑ=vaÿÑCÐø}?ŸOç?ãÑ³Ï�Ñ=JÓFóÄtó×ãA¿_N'\Nà4pYÇ#�TQÐñ54ÑQ_í¹×?�FùÓ°ßP1E Ôqƒâ}â÷Ï}AßœsøÝ�àÿÑ„±ö6õ¯?ÿÿ—)àñO:”<"2¢æbš¯}š<º Ö!ðWàþ.ºM¯�«þìõAcP¸¡bÈPSJû�NºuuË‰1þ£CLÿßƒûŒ=T÷"eß�PÃuÿH÷(„ d¢Dµ`îûS&µÔ…?E	ÿh˜á¸ì#v:�ˆ©,B•UË�ÿñ}À‚«ê]ÆfuöM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¸11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛPÅëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R_0?¼eYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾½±�+i�Ó4TN E¢¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pÍA`��A3õÒÿÃÜãßg÷<WS;a?ƒñºÿl!‹fC=5ó~ ÏcÃÜc/‘i/ZŠTt´‚ô;’¨{]fßNù‘'ÙVU¦¤»ÑU    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7249" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6458-7249" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7250-7318" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5699-6287" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4635-5149" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7319-7423" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5345-5351" color="#ffffff" type="standard"/><Segment range="5352-5369" color="#ffffff" type="standard"/><Segment name="-35" range="5370-5375" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5390-5411" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5321-5337" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1831" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26914.03" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3855-3857" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3858-3860" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3861-4574" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="IRES" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3291-3843" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ¹<?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Primer 1" sequence="GTGGTACCTCACCCTTACCG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite><BindingSite simplified="1" location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>fa2a8599-04f1-4ed3-adb0-7ad0f329b615</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="5:14:32">2022.6.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ³<StrandColors><TopStrand><ColorRange range="3261..4573" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="3261..4573" colors="magenta"/></BottomStrand></StrandColors>  ÿ      Ú           XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡°]ü<×ørýë <ªˆ?„¾U¿*Aî �ÿúDˆ+‚AEùWJüûË…ªŽ¬*ñO3ÌË¢ã�6Êâ(»‹‡ˆ«‰¨¸U˜Àäø§Þþ”¨~�·�Q‹ÎÝ},$bÔQ )úã¸Ñ`õú—,<Äpß©|Gí·ô)FÔ.«~Í�ßÑô/ùÛ!ø>ƒªÿ¿€µ?¨�þSï>û¯½¼ÿ.ïþ…ûì¬Ï±¾þ¦ôþÞúîC?ö`öa¦`ÿÏú%zþáØuž]?îïS·§C»ž’ôÜRàGî4ùõ^ÇÂ	û§Ä°ÇtˆÏôyEùìàT§t¾8}ÿß?µI6Í:w<ýÈPãÇü �öû“ûN�}€‹ùý;à;ãˆ|sÛŒvôaýøôø¹ôøGH}½"ÐôÄ¹îƒ ›Mü5ûN8npÏL~42>MÓ0ïý'O…}64|AÄ‰ë�MCáùþEúÁ7Ôà^ÁHÁàÃ;…#N4ÒàÎ‚ðø7“!N/�B/wÛ~¹çczÈSD=@øÃE±þq}IÓJÔ]CßáóÇ}4×|ùøS‹“áûÄóßž¨Ûœ0çž±ç€=!‚ß"ôÛ¶'ÿïçàuÄE5Ã}¶÷mQTßˆ÷DÓýN+€þ ƒÃ#Šâœ>8-Òâ2�õÓEÑ=vaÿÑCÐø}?ŸOç?ãÑ³Ï�Ñ=JÓFóÄtó×ãA¿_N'\Nà4pYÇ#�TQÐñ54ÑQ_í¹×?�FùÓ°ßP1E Ôqƒâ}â÷Ï}AßœsøÝ�àÿÑ„±Â|Àüñ•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVöÆ­¦LÑQ8ˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃ5‚65`Ï×Kÿp�}ŸÜñ]Lí„üROÆëý°†,1™ô×Íøƒ<!�qŒ¼2D1¤½j)QÐfÒÐîJ¡íu›}8SæDœOdeYTjš’ïET    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  í<?xml version="1.0"?><Features nextValidID="9"><Feature recentID="8" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1831" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4012-4028" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4081-4102" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4036-4042" color="#ffffff" type="standard"/><Segment range="4043-4060" color="#ffffff" type="standard"/><Segment name="-35" range="4061-4066" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6010-6114" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3326-3840" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4978" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5940" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5149-5940" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5941-6009" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  ¹<?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Primer 1" sequence="GTGGTACCTCACCCTTACCG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite><BindingSite simplified="1" location="1610-1629" boundStrand="0" annealedBases="GTGGTACCTCACCCTTACCG" meltingTemperature="58"><Component hybridizedRange="1610-1629" bases="GTGGTACCTCACCCTTACCG"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>bee05c3b-baef-435a-8907-199bc0923c50</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="1:42:20">2022.6.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  |ý7zXZ  æÖ´F !   t/å£áÅ :] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷Ànæìð�E
%„ˆp[áÄÏÀ†Ò‡ÝíÃŠ£3Ý¯çò�k¨ÏâLjj'ˆ6•{"ŽöœŽ¤qŒ�%ÝÖÓ»Ùvd¾îI4@P')²l˜s±ý}®”º£º½”�©*ºØ5Qœ<Â­kòó–È2�›[i„LüU«ƒâ13½œ<}[nÀ*nÀ}?;PúKE©æ�ºáEÒÅ�ƒc£\¨±É8ÚUúZPÞ{W”'¢úJRáÈ!e8®ÞTòàNÔi¤»ÂÌ¹·üý M%=ðvé—–!ƒZTMA¸nm„Eg&Ás#’æª-çƒîÜµú;.žèW¾˜­›c“C¶ñ”�Z ýÀŽLõ°ª| j*©õ_ÆAåŽ_áÙ†/Ž7„Ñ4l¶eQ~‚E:eí›K¬»æÖœ¥ØQ£8~TÆö(úQÎL«X´él_4`ñäüx‚/ôÀÇ•Œ§eSfÔò†ÁÐcð–e*tˆ™¦#ÖÆr¼Ž€C{‘umï¥U¶Ÿ¬!ÙîSƒù€¹¬Òm°!é~¯¬ÈÊÇYDÍ—ó€T ¥�d¸¹oiÑKæ"ê¤wîk›UHr)O¶¯Hix´þ-æ†‘�Ún#÷fß°*ã¾#íÚˆÀ*XàÃ¥¯`ÚŠ]‘ß� +v‡Gv–dsÕ³¨BýÃáxÛãRSÑy<²¬MÚÆÛ›3Ÿ©9ÝíMÜe1w`”[¬Xà!p�
$Ë÷k\‚¤0Ø0FÅº”"Ù¶s«¤E  ùµDgã	4/^tvÔé¥ÈW¸l&rMïw+�¢ò3Qé4‡†<ûßŠ¦–óO‹Ù–ð&M·! TŒìë¤¡óòÝaÄçZ¾,ZTÉcç‡/mU"ó“r;.t�%ìÇy`>®Œ­áû_ÚÊ“Ç»ãÜühy²áeÜ×uµS9±á'BLò²åf#LŽÞ)Údý+vuÍ[:@.Æoy½‹_·H3Àÿ»]K#³
2EË!–‚™ÿ¼â¾Lšä’õ4¯„ú¶è ù/À	 �ÕóÔžt—3ìÚ’3ªŠÉZn³O.keœT÷I­ëZ"ÃßÖe),ePAz¬:°+ø5êfóÝ—«$ þöÌ	·ñÎý·Å|ÿ¶LÚR
Q É‡V,€¥èWL’·7Ù­ÓrV1ËŽu—Ðn:ÛƒõüpiØž|1¼�¯Z‰¼©<mKJ*ö¾Œÿ�káÍ¼¥¢Íß Cò}ñå†½ÁlÛg
Ý+5±ÍU
&-­S,&ÝÇ™¦\bdmª<ão-/“te1ì!WAðƒD®Í¸	-â@B³½K‰òÇ×@:Îf	¬QóS±‹ì<ù|Õ­(êó	È\�âù½ÿN7i�`RûcÙô‰‡º~/>cð8Q¾
â|8ì�n§ëK¹I
»»>›ÆS ö”0Ú§Þ¤…\E(lÚBM=!½[sŒ:¿KHFªó«=2ÐK|$ÑOËœ1ì‡‚›áá�è¤ó|}ãèeõîˆ›þÕzz˜ {¥ßóPïN_ù Ð&&w»°ÓGãab9§�Œúºs…ïÆDW-Ô‘ë¿Û!„ëöW¼ŠÍ¬„ƒ#ÇvˆáTý~ðÝµn¨u&D4ÉÙâÿ2æ…‹ã¥l
“>XÉžôþó��íd�¥+wKfêW,cõ©]ykÆ µŒìvµRwâ
9£ ¬§ñÙõÓ¡®ýÃþg3Óði–¶Å%3¼áô?!–}ûy¢ 5÷¨{»»«èÑ‚bMÎ}]ûÙ�ôÓ'Ó^8wI¢m¶ Qp&é0¥Ý‚
VúËƒï+ûJÎ«o>U²9e¼7g‹™(k‘ïc£ÎÍ‘
Žøž-k4ßD‹Îoi8÷6ã%â¹Cò‡áž@s[EpBÎÆ|þ›%ÚŒ,t›Ed.ƒ*]'Ã3 XA²Ìršl’yÏd 9pˆÖøØ¸ÈÞÐE„4gÖç7=~–Ü°§2ÆËZéóAÄÍ˜qÏ~‹W�Î—!5Ö€“Ql© wÑ|ìCrn¤ôýSQÌÿ- <+Ü§Y®rá„sïË`Új ÍY6ƒ˜òË¨à}à+¬´Ùo'¶�¼«¸'�â$Ç0Ê}¬ùÇ!» ê|g£lô´zhn"®Ë6ìçÁøQQx6¬6ÕYU<"tÝ}ë¦ý»|¨JXôf¹G›!j¯Y[…AOE¤©qd{ƒÇ¿’K$ð]¨)bô8-Q—z­êgð®Š²´žKÐdV¶j³5jõæ¸3#qÝ\¾ãMm#UïïôCÜ@9Î‡ÒÞƒH­cZÿåÆ“-@N»¯Vƒ‚I /z[Lå¢C¥ÌøU$sŽa\6a²oER*ÐT¨-oçõ.ŒÜßYó™vÝeà©¥ ÈmÕu°å×b´NOÇÞý`Ý‘dÐVÇ÷ÀSü�f0©uâï˜·-;E5G�±µµpsQ¡žQï(ëÄãÙsAŠ/æú©G¦Ý¢Au$ÿXÈdwÂßÇPôlø�ˆR)SUKC§<ÓW7(gŒäW#®Ž¡S€’„¶¦Æˆn>½¸[[~,Ë®©‹ýá¢¤sSUR©–‚ÕÈÚI NžÃfãJgš ¸H8ÚL Áëï0'=¬PŸ;DUø[;.Ä¾½&dZ|,åÌ’0ŸcûŽ†÷Yi‹”“‰¼;È¾=¥ ?Z‘Xf¡7c®@P9³ÿÌ?5ü?-†	¼lY ŠnV«¬p›�Ò½ôÈ�dèí•‰ RøWzÙ$Žýpœ×‚©¢@K¬)Aü·õ{›æb� 	ž#�ôÝ‰ï‚x}.éiº…Í‚ y±–Z�•LyæïG¯Œ±åš¼rBéÉ;#ù" ÅW¹›cj¬, ¡×Í‹�÷. \l¶™©jÇ€	�)˜öM¢£ÿŒóÍÚ©¬‘ú¹uÿ¢+%bˆÞõâ?ýŠš¡6Ä'€K„²«úðýj»T�séQ¶Ø1w¨dý_¢8$J¹�3,8\jW”¼\2Mh¯Èz€¸ôq{ûžóBÛYÓb^ßxs;ûN)Ø�5_í×1É’Á¨R&€syÌÈYï}¨íÕCçt&P¹­Z¢ñTK—£wß$Ä%~1&uÏZª×¥%âªZÚÎ‡â=ª+›DÌ�¢+A¼:Ê˜íÑ:€(÷âIÿÒÓ+Ërs§èÛcc¸×þù ›¥.Üú’r…¸Y”cr7ªa<w�…À MM•-*ÓKÑß¨C<¤+kûƒ4Plæý‡Dd5HU‡û;Ö4{#SM>AdË
U�£ÁÙl)c.eÑ6YÀR�=Àyº¥Ã~Š|&*6¤ÀORÔ²%0WÉ¡f@¡)”û+Aª¨íæfOXpíc_dÈ—�¡!p—„¥W!þ+í?=¬ÀêðÑ]ŸÞ^üéÏË©7NÆ8÷½¢4›–to›Ù£y‹£å	Ú{^!â(ô4ú%÷�Šê^qô}±…Ç~Nç
ý±·B’,n¶ÁC€�íµò
¤¬Gj�ƒÒK«nµý&Œ£Ê§¼7ÿx2ª²W—ÿÑh%]SÂÄŸÜ&£óíÝ*ÐÜ8�_óÏ„œF=”…zBÙ¿ÏR¤ ž–„­«ÅW4þ¨?¢½D¸1�ã(šÖÊ–4¢¾æ@ë`™Qì+=w�ºgþw1º&„£CêŒò.B%ÿÂá2lMbûÈaÍ(£�Ž`¾õÙ+ê 1Í§Ì�‹°'#–³4ª$s'„­d~æIAJ†2<À1Ü\'E°¡ÓéY‰ã5êI„#Cì¥€�ÁÝçrÆoý
cÎºÇûÉ:ÇH%hw«¯¥�Â²ÿ”u¤ïê:Ðãy�‡$Ú©w6T­=® êl¥ƒ’¼ØS&ßø½[�^n¦žCõÉs(Õ×$8’ 9äÜzžN)ýƒò�ÏwœÀ¯á¯ >_B ]‡ÄÐÞÔðÏôë­>�=,¼öèIàZ XxL‹’»B-)ž�Óoò:ç|Î�Ø2@öó7×¥�ÊlÅY+GQÝÉöÃ¢Æºµ3ÔP•n<j©ðöøþÊ/*€ª–zÂü=gÿŽ›�'¤8 45L"*ââÈ€\~DE]\U¶ûf îBô]Ö“iñxqC%LÊj6.…¾„µÇ¾K©3å…›ÙÄ¯ìœQŽ¸)«†šw©â8
�uÇ©~¾}÷uD4 ç·^ø¶±	»bÌìOŒAáüããÛ^¯õÛ­:Ïâi¢ç£É_ôíÀ¬ìjÓQ�L EL²�¦�(‘Öö,ÈjôpÇÝ·³æ¶"}sæBÊÝJ]1Á_–ÜºÏj% ¾�úè#"H£dh@ÖT�ÒlÆèÄ€Ì
 ¯!]TQ�;c�sOD7tîà!É²\‡·1�ŠÂP«íØ¿\ärsn¥L”ãÖoûu9€¶=<„†vk¼ˆÎ:vBçìšXü—I ëå’‡|±3Š™iéKü8Eû¿�"må„Üf¢ÑŸ1~üÇÄ{JÓÚéÍýô9û+Ðå³_ð/î@{$‹~–Ý–‚æ7ÑàèÔÐPÀ<
PÀ °
½X‰áó•F’*ö|Nð½œZÊpØ‘,£ÂL¹)ïŠ¹G'Z"ÎôLlpxYW—4X×XUÙ87nüØ½	®Z°xáv®#Dœðâ+uˆÿ¡årèÚëØ~-
¸Oò3C®ÝþÕ2î{ÎÇ!S¦Ò §õß0|Û>Ù¥i·3¤ytP: £gÇ„œôsïƒ>·îƒð;ŒAÃ]™ï®�í†ù’‘®Á0Ž]~aÝÕoV
C"$ÿ–u˜zR…À'äøê4´Ù¢Êj“ÃÊLîòXCeüaà¸kÞ_ÿ6q¦Yÿ’xâ“ê�–YžÃ‚J¾!Ú¼¨ªq'Ñ{â±iÐ�œ Íg€ÚîK‹"X”4ú½<«Ia5º£ð
„(ÃP»Ç
pñn|šbY¥UéJú6¤s¨Ñr�G�,S!©ëý)¶?SVHœ«d¶8rËkòy ±|%šÃÀnq<|æªîr_‡: ËSÁŠjtáÏJyÈæ<8Õòî”/ø;Šd·�s^ð¦:YB˜`˜Þ3–£~:´F
#—V$Ž¹¡ãZÚó_=‡«VIJu/çž“Ú¹,Ù½ç|kéoG�Ç©z�E¢xÿOw…òQˆ”ã°ØVCc”Þc„N8í¡åÄ}ø/ûTbt¥%†Ò˜£·>;«D»IäM{M#8�ÌB3xMÀö�#ùXF÷OŒç[*Ž²Í¿ÐÊ¢ºíF»/›	ÅÐå ; ¼OäK"c¶Ãhý÷„U«Q·N¦aA T†ÚÏ7MuKc¾H×µ{4ÛÌb?¹û.Ëí-ØËU¦~ò1×œÎ¡g0´Îry¹ë´Tyüù×Õ³‹ÉJ$~&³�<LŽUD"˜Ë¢‚Q(zE! ìáfÔÖ�Ó0ÉéÃGÁ CèèÄ°çðUÐ£Y#$üÒ =IR�˜uÂô¦Æüõùå—àx#æN—ÃÓý!®¿àÄr ±t¢HÂÊ˜rG€
O§þ‰¨Z²ì’p½³èLST¦®�¾>)„ÌÆ~%ƒQÅéÍh‚\p¤×6éÓ�¦sˆpBÓ•wbÎB³2¡Üi  tõùÕ¡É¹œÃ´óiî$K°˜3ï;u ¤Ð8?‚“c�ÇûÈU(ÖE_FízÀµ0¢B³%B]/­Ô‹M[„Û}åð]´Y'8ŒI“ 
µÐËà&K£¦:í¹¢pð‚€Ä™Ÿˆà~ÂeçÄÆýXfÊhµZv¿Œ÷�ˆÁuó÷÷Ëô¸r%Ã­Ë-•hi³Ùêæ&½Êª¹¿ëÔãö'¯ÕÇ?˜è ß9Ž¯{â¢l»:�³TûÈ‚µCàÌ�ã`qÏ±U²Sn h`£�Ç—}b²e´>tßºQ)›æ[!6«� “¬UÁ¼ÜV�OŽˆ»Ö¤éÔÉÑN¬ÑP_!øÜëTÚgrÇï‹ð    Êä_ò| Ö$¡Š ÚÔGe±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  4<?xml version="1.0"?><Features nextValidID="23"><Feature recentID="0" name="MCS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7129-7148" color="#ccffcc" type="standard"/></Feature><Feature recentID="1" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2914-2930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2840-2861" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="2876-2881" color="#ffffff" type="standard"/><Segment range="2882-2899" color="#ffffff" type="standard"/><Segment name="-10" range="2900-2906" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="828-932" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8447-8963" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2552" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1001" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="933-1001" color="#ccffcc" type="standard" translated="1"/><Segment range="1002-1793" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="8" name="P2A" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6397" maxFusedRunOn="6397" detectionMode="exactProteinMatch"><Segment range="7118-7122" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="A"/></Q></Feature><Feature recentID="9" name="EGFP" directionality="2" translationMW="26942.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6397-7110" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="7111-7113" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="7114-7116" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="10" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3895-3904" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="11" name="Factor Xa site" directionality="1" translationMW="473.54" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5836" consecutiveTranslationNumbering="1" maxRunOn="6220" maxFusedRunOn="6220" detectionMode="exactProteinMatch"><Segment range="5825-5836" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="12" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3628-3657" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3658-3687" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3688-3717" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3718-3747" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3827-3858" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5597-5718" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="WPRE" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5719-6307" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3901-5553" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"/></Q></Feature><Feature recentID="20" name="3' LTR" type="LTR" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3102-3616" color="#ffe4c4" type="standard"/></Feature><Feature recentID="21" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-3777" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="NFAT binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3778-3807" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;NFAT transcription factor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;NFAT binding site from the human interleukin 2 (IL-2) gene (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/1702384&quot;&gt;Mattila et al., 1990&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>8ab407f2-9c85-45ed-a7fc-30727163ccee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSGV-MCS-EGFP-6Ã—NFAT-minP-Fluc</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="0:51:49">2019.2.11</Created>
<LastModified UTC="3:14:19">2026.4.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..3625" colors="red"/><ColorRange range="3626..3806" colors="purple"/><ColorRange range="3807..3893" colors="blue"/><ColorRange range="3894..5549" colors="green"/><ColorRange range="5550..5556" colors="red"/><ColorRange range="5557..5717" colors="green"/><ColorRange range="5718..6306" colors="orange"/><ColorRange range="6307..7084" colors="gray - 50"/><ColorRange range="7085..7116" colors="purple"/><ColorRange range="7117..7145" colors="red"/><ColorRange range="7146..8776" colors="gray - 50"/><ColorRange range="8777..8962" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..3355" colors="black"/><ColorRange range="3356..3586" colors="gray - 50"/><ColorRange range="3587..3625" colors="red"/><ColorRange range="3626..3806" colors="purple"/><ColorRange range="3807..3893" colors="blue"/><ColorRange range="3894..5549" colors="green"/><ColorRange range="5550..5556" colors="red"/><ColorRange range="5557..5717" colors="green"/><ColorRange range="5718..6306" colors="orange"/><ColorRange range="6307..7084" colors="gray - 50"/><ColorRange range="7085..7116" colors="purple"/><ColorRange range="7117..7145" colors="red"/><ColorRange range="7146..8776" colors="gray - 50"/><ColorRange range="8777..8962" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
